get_extcoeff_A280: Get a protein's molar extinction coefficient at A280

In ec363/fpcountr: Fluorescent Protein Calibration For Plate Readers

Get a protein's molar extinction coefficient at A280

Description

Work out the theoretical molar extinction coefficient of a protein at 280nm (EC280, M-1cm-1) using only the protein's primary sequence, using the ProtParam method (Pace values). A full explanation of the method can be found at https://web.expasy.org/protparam/protparam-doc.html.

Usage

get_extcoeff_a280(
  protein,
  disulphides = FALSE,
  showWarnings = TRUE,
  showMessages = TRUE,
  protein_name = "-",
  buffer = "-",
  mol_weight = NULL,
  save = TRUE,
  outfolder = "."
)

Arguments

protein	character string of protein sequence using 1-letter code
disulphides	logical. Does protein have disulphides?
showWarnings	logical. Should function show warnings?
showMessages	logical. Should function show messages?
protein_name	character string of protein name. Optional.
buffer	character string of buffer. Optional.
mol_weight	numerical value for molecular weight (g/mol). Optional. If specified the function gives extinction coefficients for 1% (10mg/ml) and 0.1% (1mg/ml) solutions too.
save	logical. Should function save CSV file of output?
outfolder	path to folder where output files should be saved. Defaults to current working directory.

ec363/fpcountr documentation built on Nov. 29, 2024, 12:03 p.m.