create_colkey_4diln_2ctrl_in_24: Create a 4-dilution column key for primer calibration

In ewallace/tidyqpcr: Quantitative PCR Analysis with the Tidyverse

Create a 4-dilution column key for primer calibration

Description

Creates a 24-column key for primer calibration, with 2x biol_reps and 2x tech_reps, and 5-fold dilution until 5^4 of +RT; then -RT (no reverse transcriptase), NT (no template) negative controls. That is a total of 6 versions of each sample replicate.

Usage

create_colkey_4diln_2ctrl_in_24(
  dilution = c(5^(0:-3), 1, 1),
  dilution_nice = c("1x", "5x", "25x", "125x", "-RT", "NT"),
  prep_type = c(rep("+RT", 4), "-RT", "NT"),
  biol_rep = rep(c("A", "B"), each = 12, length.out = 24),
  tech_rep = rep(1:2, each = 6, length.out = 24)
)

Arguments

dilution	Numeric vector of length 6 describing sample dilutions
dilution_nice	Character vector of length 6 with nice labels for sample dilutions
prep_type	Character vector of length 6 describing type of sample (+RT, -RT, NT)
biol_rep	Character vector of length 6 describing biological replicates
tech_rep	Character vector of length 6 describing technical replicates

Value

tibble (data frame) with 24 rows, and columns well_col, dilution, dilution_nice, prep_type, biol_rep, tech_rep.

See Also

Other plate creation functions: create_blank_plate(), create_colkey_6_in_24(), create_colkey_6diln_2ctrl_in_24(), create_rowkey_4_in_16(), create_rowkey_8_in_16_plain(), display_plate(), display_plate_qpcr(), display_plate_value(), label_plate_rowcol(), make_row_names_echo1536(), make_row_names_lc1536()

Examples

create_colkey_4diln_2ctrl_in_24()

ewallace/tidyqpcr documentation built on June 5, 2024, 10:04 a.m.