create_colkey_6diln_2ctrl_in_24: Create a 6-dilution column key for primer calibration

In ewallace/tidyqpcr: Quantitative PCR Analysis with the Tidyverse

Create a 6-dilution column key for primer calibration

Description

Creates a 24-column key for primer calibration, with 1x biol_reps and 3x tech_reps, and 5-fold dilution until 5^6 of +RT; then -RT (no reverse transcriptase), NT (no template) negative controls. That is a total of 8 versions of each replicate.

Usage

create_colkey_6diln_2ctrl_in_24(
  dilution = c(5^(0:-5), 1, 1),
  dilution_nice = c("1x", "5x", "25x", "125x", "625x", "3125x", "-RT", "NT"),
  prep_type = c(rep("+RT", 6), "-RT", "NT"),
  tech_rep = rep(1:3, each = 8, length.out = 24)
)

Arguments

dilution	Numeric vector of length 8 describing sample dilutions
dilution_nice	Character vector of length 8 with nice labels for sample dilutions
prep_type	Character vector of length 8 describing type of sample (+RT, -RT, NT)
tech_rep	Character vector of length 8 describing technical replicates

Value

tibble (data frame) with 24 rows, and variables well_col, dilution, dilution_nice, prep_type, biol_rep, tech_rep.

See Also

Other plate creation functions: create_blank_plate(), create_colkey_4diln_2ctrl_in_24(), create_colkey_6_in_24(), create_rowkey_4_in_16(), create_rowkey_8_in_16_plain(), display_plate(), display_plate_qpcr(), display_plate_value(), label_plate_rowcol(), make_row_names_echo1536(), make_row_names_lc1536()

Examples

create_colkey_6diln_2ctrl_in_24()

ewallace/tidyqpcr documentation built on June 5, 2024, 10:04 a.m.