reducedFlowframe: generic function for extracting the full flowframe

Description Usage Arguments Value Examples

Description

generic function for extracting the full flowframe

Usage

1

Arguments

x

an object of either class PhytoFilter, MarginEvents or DebrisFilter

Value

generic to extract fullFlowframe

Examples

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flowfile_path <- system.file("extdata", "B4_18_1.fcs", 
package = "cyanoFilter",
              mustWork = TRUE)
flowfile <- flowCore::read.FCS(flowfile_path, alter.names = TRUE,
                               transformation = FALSE, 
                               emptyValue = FALSE,
                               dataset = 1) 
flowfile_nona <- cyanoFilter::noNA(x = flowfile)
flowfile_noneg <- cyanoFilter::noNeg(x = flowfile_nona)
flowfile_logtrans <- cyanoFilter::lnTrans(x = flowfile_noneg, 
                      c('SSC.W', 'TIME'))
cells_nonmargin <- cellMargin(flowframe = flowfile_logtrans, 
                              Channel = 'SSC.W',
           type = 'estimate', y_toplot = "FSC.HLin")
 reducedFlowframe(cells_nonmargin)

fomotis/cyanoFilter documentation built on Aug. 1, 2021, 10:58 p.m.