BoxPlotMarkerMetaClust: Box plot of one marker MFI, for the different meta-cluster...

Description Usage Arguments Value Examples

View source: R/BoxPlotMarkerMetaClust.R

Description

BoxPlotMarkerMetaClust is a function that compares, inside each metacluster, the median fluorescence intensity of one given marker for each experimental condition.

Usage

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
BoxPlotMarkerMetaClust(
  TreeMetaCl,
  Title,
  treatmentTable,
  ControlTreatment,
  BottomMargin,
  Marker,
  Robust = TRUE,
  ClustHeat = TRUE
)

Arguments

TreeMetaCl

FlowSOM tree with meta-clusters, constructed by buildFSOMTree

Title

prefix of the pdf file name

treatmentTable

data frame containing a column 'files' and a column 'Treatment' (case sensitive)

BottomMargin

size of the boxplots bottom margins, in order to be readable

Marker

marker name

Robust

if TRUE, robust statistics is applied (dunn), otherwise linear-model/Tukey

ClustHeat

if TRUE, heatmaps are clustered

Controltreatment

name of the experimental condition that you consider as your control treatment used for statistics

Value

list containing MFI, pairwise comparison p-values, controle vs treatment p-values

Examples

1
BoxPlotMarkerMetaClust(TreeMetaCl=CytoTree, Title="Experiment1",treatmentTable=Experiment1table.csv,ControlTreatment="control",BottomMargin=3,Marker="PD-1",Robust=TRUE, ClustHeat=TRUE)

gautierstoll/CytoSOM documentation built on May 16, 2020, 10:36 a.m.