GeneUpDownStream: Get Genes plus Up and Down Stream Regions

In genomaths/MethylIT: Methylation Analysis Based on Signal Detection

Get Genes plus Up and Down Stream Regions

Description

Given a genes region or genomic region (GR), this function yields the GR plus the especified amount of DNA bases upstream and downstream the GR.

Usage

GeneUpDownStream(
  GR,
  upstream = 0,
  downstream = 0,
  extend = NULL,
  fix = NULL,
  onlyUP = FALSE,
  onlyDown = FALSE
)

Arguments

GR	A GRanges-class object containing the ranges of the genes or genomic regions to be extended upstream/downstream
upstream	Integer (Default: 0). The amount of DNA bases (bps) upstream of the GR.
downstream	Integer (Default: 0). The amount of DNA bases (bps) downstream of the GR.
extend	Integer (Default: NULL). If upstream == downstream, then simply you may use extend.
fix	A string with one of the three possible values: 'start', 'end' or 'center' to denoteg what to use as an anchor for each element in GR.
onlyUP	Logic (Default: FALSE). If TRUE returns the region upstream the GR.
onlyDown	Logic (Default: FALSE). If TRUE returns the region downstream the GR.

Details

Users can select whether to request only upstream, only downstream, or both, upstream and downstream. Please notice that for a gene on the negative strand, 'the start of the gene' corresponds to the 'end' of the gene in the GRanges object and the 'end of the gene' correspond to the 'start' of the gene in the GRanges object.

Examples

starts = c(65419, 450703, 923928, 944204)
ends = c(71585, 451697, 944581, 959309)
chrs = c(rep('chr1', 2), rep('chr2', 2))
gr = makeGRangesFromDataFrame(
        data.frame(seqnames = chrs, start = starts, end = ends,
                strand = c('+', '-', '+', '-'),
                genes = c('A', 'B', 'C', 'D')), keep.extra.columns = TRUE)

gr1 = GeneUpDownStream(GR = gr, upstream = 2000, downstream = 1000)

genomaths/MethylIT documentation built on Feb. 3, 2024, 1:24 a.m.