LBE_fluo3D_processing: 3D Fluorescence spectrum processing

Description Usage Arguments Examples

Description

This function treats your set of ".sp" files withou affecting them. You just have to set the R's working directory to your folder containing your .sp files of a 3D spectra. Results (tables) will be exported to a folder inside your directory called "Output_data" .

Usage

1
2
3
4
5
6
7
LBE_fluo3D_processing(first_spectrum_line = 55,
  excitation_spectrum_line = 22, excitation_start = 200,
  excitation_end = 590, excitation_step = 10, export_3D_matrix = TRUE,
  increase_resolution = FALSE, new_excitation_step = 2,
  emission_start = 200, emission_end = 600, emission_step = 0.5,
  remove_max_height = FALSE, max_height = 800, replace_max = NA,
  remove_difdif = TRUE, replace_difdif_by = 0, directory = getwd())

Arguments

first_spectrum_line

Normally you shouldn't change this. It is the first line containing the spectrum in the .sp files, 55 by default

excitation_spectrum_line

Normally you shouldn't change this. It is the line containing the selected excitation in the .sp files, 22 by default

excitation_start

Default = 200. Only change if you are not using the default scan3D method. Otherwise, it is your first excitation wavelenght (nm).

excitation_end

Default = 590. Only change if you are not using the default scan3D method. Otherwise, it is your last excitation wavelenght (nm).

excitation_step

Default = 10. Only change if you are not using the default scan3D method. Change to 20 might be useful if you are running the fast method. Otherwise, it is your excitation step (nm).

export_3D_matrix

Default = TRUE. Accepts TRUE or FALSE. Use it to export or not the processed data matrix into csv files.

increase_resolution

If you want a more beautiful plot chose TRUE to increase the excitation step from your original to new_excitation_step.

new_excitation_step

See increase_resolution. No effect if increase_resolution is FALSE.

emission_start

Default = 200. See excitation_start for details. Your first emission wavelenght (nm).

emission_end

Default = 600 See excitation_start for details. Your last emission wavelenght (nm).

emission_step

Default = 0.5. See excitation_step for details.

remove_max_height

Default = FALSE. Pick up TRUE if you want to replace all values greater than max_height by replace_max.

max_height

See remove_max_height. No effect if remove_max_height is FALSE.

replace_max

See remove_max_height. No effect if remove_max_height is FALSE.

remove_difdif

Default = TRUE. You can chose if you want to remove the diffusion and diffraction peaks and the outside areas from your plot.

replace_difdif_by

Anological to replace_max. No effect if remove_difdif is FALSE.

directory

Default is the working directory. Better not change it.

Examples

1
2
> setwd(XXXX) #XXX is your directory contaning the .sp files (copy and paste from the brownse and replace all the "\" by "/").
> LBE_fluo3D_processing() #include your options if you want.

guilayn/lbe3df documentation built on May 30, 2019, 7:20 p.m.