Description Usage Arguments Details Examples
IMPORTANT:
1 2 3 4 5 6 7 8 | LBE_fluo3D_process_all(plot_all = TRUE, exci_spec = FALSE,
emi_spec = FALSE, indic = TRUE, zones_calc = TRUE,
zones_polygons = "Default", args_process = list(increase_resolution =
FALSE, remove_difdif = TRUE), args_volume = list(zones_polygons_ =
zones_polygons), args_plot = list(zones_polygons_ = zones_polygons,
plot_zones_polygons = TRUE, export_html = TRUE, export_png = TRUE, export_pdf
= TRUE), args_exci = list(), args_emi = list(), args_indic = list(),
all_directory = getwd())
|
plot_all |
Default = TRUE. Use FALSE to avoid plotting. |
exci_spec |
Default = FALSE. Use TRUE to include this function and specify emission as in excitation_spectrum() |
emi_spec |
Default = FALSE. Use TRUE to include this function and specify excitation as in emission_spectrum() |
indic |
Default = TRUE. Will include calculation of indicators to every spectrum. |
zones_calc |
Default = TRUE. Will include calculation of volumes and barycenters of each zone to every spectrum. |
args_process |
List containing the conditions for the function LBE_fluo3D_processing. See individual help files for details (?LBE_fluo3D_processing) |
args_volume |
List containing the conditions for the function LBE_fluo3D_zones_volume. See individual help files for details (?LBE_fluo3D_zones_volume) |
args_plot |
List containing the conditions for the function LBE_fluo3D_levelplot. See individual help files for details (?LBE_fluo3D_levelplot) |
args_exci |
List containing the conditions for the function excitation_spectrum. See individual help files for details (?excitation_spectrum) |
args_emi |
List containing the conditions for the function emission_spectrum. See individual help files for details (?emission_spectrum) |
args_indic |
List containing the conditions for the function LBE_fluo3D_indicators. See individual help files for details (?LBE_fluo3D_indicators) |
all_directory |
Directory containing subfolders with .sp 3D fluorescence files. Default is the directory used by the user. Might be useful to manually specify another. |
1) EVERY SUBFOLDER MUST PRESENT THE SAME CONFIGURATION OF SPECTRA (ANALYSIS METHOD), FOR A SINGLE SPECTRA Reminder: The default internal method results in 40 .sp files called XXX#01, XXX#02, ..., XXX#40 where XXX is the label defined by the user.
2) THE CODE WILL DETECT AND STOP IF THERE IS ANY SUBFOLDER THAT DOESN'T SATISTIFY THE CONDITION ABOVE
1 2 3 4 5 6 7 8 9 10 11 12 13 | # (!!!) First of all, verify the conditions hilighted in "IMPORTANT". (!!!)
> setwd("C:\\XXXX") #where XXXX is your directory (don't forget to replace \ by / or \\)
> LBE_fluo3D_process_all(exci_spec = T, emi_spec = T,
args_process = list(remove_difdif=F),
args_plot = list(plot_zones_polygons=F,color_palette=2),
args_exci = list(select_emission=327,export_png=T,export_pdf=F),
args_emi = list(select_excitation=328,export_png=F,export_pdf=T),
args_indic = list())
# The code above will plot the gray spectrum (color_pallette=2, see ?LBE_fluo3D_levelplot for details), for all subfolders of XXXX.
# It will not remove diffusion and diffraction peaks and outside areas (remove_difdif=F, see ?LBE_fluo3D_processing for details)
# It will also plot excitation and emission 2D spectras for 327 and 328 emission and excitation wavelenghts, respectively.
# Observe that emission 2D spectra will be exported as png while excitation will be exported as pdf (just as example).
# By default, all the available indicators will be exported into each subfolder and a summary in the master folder.
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