NormScoreCC: Normalize counts and score cell cycle for each cell

In j-andrews7/EZscRNA: Simple Convenience Functions for High-Level scRNA-Seq Analysis

Description

NormScoreCC returns a Seurat object with normalized counts and adds cell cycle scores for each gene based on Seurat's cell cycle gene lists.

Usage

NormScoreCC(scrna, skip.sct = NULL)

Arguments

scrna	Seurat object to score cell cycle genes for each cell.
skip.sct	Boolean indicating whether to skip SCTransform call. Useful for integrated/already normalized objects.

Value

Seurat object with cell cycle scores ("S.Score", "G2M.Score") and "Phase" added to meta.data for each cell.

Author(s)

Jared Andrews

Examples

## Not run: 
library(Seurat)
pbmc_small <- NormScoreCC(pbmc_small)

## End(Not run)

j-andrews7/EZscRNA documentation built on Feb. 24, 2020, 10:37 a.m.