dv200: DV200 analysis

In jwfoley/bioanalyzeR: Analysis of Agilent electrophoresis data

DV200 analysis

Description

Calculate the proportion of fragments above 200 bases (DV200). Only fragments between the lower and upper markers are considered.

Usage

dv200(
  electrophoresis,
  prop.variable = "concentration",
  lower.marker.spread = 1
)

Arguments

electrophoresis	An electrophoresis object.
prop.variable	Which variable to use for the proportion.
lower.marker.spread	Amount to scale the width of the lower marker peak.

Details

Note: Despite unclear wording, Agilent calculates DV200 as a proportion of total mass (concentration), rather than a proportion of molecules (molarity). For some purposes molar DV200 may be more relevant, but current protocols refer to the default DV200 based on mass.

By default lower.marker.spread = 1 (see between.markers) for consistency with the Agilent software.

See Also

region.ratio, illumina.library.ratio

jwfoley/bioanalyzeR documentation built on Aug. 1, 2023, 4:46 a.m.