illumina.library.ratio: Ratio of good inserts to adapter dimers

In jwfoley/bioanalyzeR: Analysis of Agilent electrophoresis data

Ratio of good inserts to adapter dimers

Description

For Illumina sequencing libraries, compute the molar ratio of molecules with desirably long inserts to undesirable adapter dimers in each sample.

Usage

illumina.library.ratio(
  electrophoresis,
  min.sequenceable = 100,
  min.good.insert = 200,
  max.sequenceable = 700
)

Arguments

electrophoresis	An electrophoresis object.
min.sequenceable	The shortest length of molecules that are likely to produce data on the sequencer (ignore shorter molecules that are probably just unclusterable free primers or adapters).
min.good.insert	The shortest length of a desirable molecule (adapter length plus insert length).
max.sequenceable	The longest length of molecules that are likely to produce data on the sequencer (ignore molecules that are too long for clustering).

See Also

region.ratio, dv200

jwfoley/bioanalyzeR documentation built on Aug. 1, 2023, 4:46 a.m.