analyse.primers: Plot Curves For Testing qPCR Primers

In kiefer-ch/qpcRo: qPCR

Description

Plot Curves For Testing qPCR Primers

Usage

analyse.primers(scheme, dil = NULL, notPass = NULL)

Arguments

scheme	A pipetting scheeme, e.g., generated by get.pipettingScheme. Generally a data.frame containing gene, cond, col and row information.
dil	Conditions and corresponding dilution steps. A named vector with conditions as names and denominator of dilution steps as values.
notPas	Character vector containing wells, that are not to be included in the analysis.

Examples

scheme <- get.pipettingScheme(paste("primer", 1:7), 1:5, 1, 2, 1, nrow = 10, first_col = 18)
    scheme <- import.LCcq("data-raw/primer.txt", scheme, decimal_mark = ',')
    dils <- c(1, 6, 36, 216, 1298)
    names(dils) <- 1:5
    analyse.primers(scheme, dil = dils, notPass = c("J22", "I22"))

kiefer-ch/qpcRo documentation built on May 31, 2019, 1:57 p.m.