bioamp: A function to analyze plot the raw data from a Bio-Rad...

In michbur/dpcR: Digital PCR Analysis

A function to analyze plot the raw data from a Bio-Rad droplet digital PCR experiment

Description

bioamp is a function to plot and analyze the amplitude data of a Bio-Rad droplet digital PCR experiment.

Usage

bioamp(
  data = data,
  amp_x = 1,
  amp_y = 2,
  cluster = 3,
  robust = TRUE,
  plot = TRUE,
  stat = TRUE,
  xlab = "Assay 1 Amplitude",
  ylab = "Assay 2 Amplitude",
  ...
)

Arguments

data	object of class what containing the amplitude data.
amp_x	is the first amplitude channel (x-axis).
amp_y	is the second amplitude channel (y-axis).
cluster	are the clusters of the plot. The number indicates the column of a table, which contains the cluster information.
robust	Is the method used to calculate the location (mean or median) and dispersion (standard deviation or median absolute deviation).
plot	logical, if TRUE, the plot is printed.
stat	logical, if TRUE, the statistics of the droplet digital PCR experiment are calculated.
xlab	x-label of the plot.
ylab	y-label of the plot.
...	other arguments passed to the plot function (see plot.default for details).

Author(s)

Stefan Roediger, Michal Burdukiewcz

Examples

par(mfrow = c(1, 2))
bioamp(data = pds_raw[["D01"]], main = "Well D01", pch = 19)
bioamp(data = pds_raw[["D02"]], main = "Well D02", pch = 19)
par(mfrow = c(1, 1))

michbur/dpcR documentation built on Nov. 17, 2022, 5:02 a.m.