ddpcRquant: Quantify droplets
In michbur/dpcR: Digital PCR Analysis

ddpcRquant

R Documentation

Quantify droplets

Description

Cluster raw data from QX100 and QX200 systems.

Usage

ddpcRquant(
  path,
  threshold.int = 0.9995,
  reps = 10,
  blocks = 150,
  threshold.manual = NULL
)

Arguments

`path`	`character` path to diectory with raw data.
`threshold.int`	`numeric` probability of the threshold quantile.
`reps`	`numeric` vector representing the number of replications.
`blocks`	`numeric` vector representing the number of blocks.
`threshold.manual`	if `numeric`, the value is used as the threshold. If `NULL`, the threshold is calculated automatically.

Value

dpcr object.

Note

This function is a modification of code was implemented using the code found on http://www.ddpcrquant.ugent.be/.

Author(s)

Wim Trypsteen, Matthijs Vynck, Jan De Neve, Pawel Bonczkowski, Maja Kiselinova, Eva Malatinkova, Karen Vervisch, Olivier Thas, Linos Vandekerckhove, Ward De Spiegelaere.

References

Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.

michbur/dpcR documentation built on Nov. 17, 2022, 5:02 a.m.