create_dpcr | R Documentation |
Creates adpcr
and dpcr
objects from data.
create_dpcr( data, n, exper = "Experiment 1", replicate = NULL, assay = "Unknown", type, v = 1, uv = 0, threshold = NULL, adpcr, col_names = NULL, row_names = NULL, panel_id = NULL )
data |
a |
n |
|
exper |
The id of experiments. |
replicate |
The id of technical replicates. |
assay |
The name or id of assays. |
type |
Object of class |
v |
The volume of partitions [nL]. |
uv |
The volume uncertainty of partitions [nl]. |
threshold |
|
adpcr |
|
col_names |
|
row_names |
|
panel_id |
|
This constructor function assists in creation of objects used by other functions of the package. It is also responsible for checking the correctness of arguments.
A warning is prompted whenever any of arguments is converted to other type.
An adpcr
or dpcr
object.
create_dpcr
is a preferred to calling directly
new
.
Currently only end-point measurements are supported.
Michal Burdukiewicz, Stefan Roediger.
Streamlined, but more limited version: df2dpcr
# Droplet digital PCR example sample_runs <- matrix(rpois(60, lambda = 1.5), ncol = 2) ddpcr1 <- create_dpcr(sample_runs[, 1], n = 30L, threshold = 1, type = "nm", adpcr = FALSE ) ddpcr2 <- create_dpcr(sample_runs[, 2], n = 30L, threshold = 1, type = "nm", adpcr = FALSE ) plot_vic_fam(ddpcr1, ddpcr2) # Array digital PCR example sample_adpcr <- create_dpcr(rpois(765, lambda = 0.8), n = 765L, type = "nm", adpcr = TRUE ) plot_panel(sample_adpcr, 45, 17)
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