flywire_xyz2id: Find FlyWire root or supervoxel (leaf) ids for XYZ locations

In natverse/fafbseg: Support Functions for Analysis of FAFB EM Segmentation

Find FlyWire root or supervoxel (leaf) ids for XYZ locations

Description

Find FlyWire root or supervoxel (leaf) ids for XYZ locations

Usage

flywire_xyz2id(
  xyz,
  rawcoords = FALSE,
  voxdims = c(4, 4, 40),
  cloudvolume.url = NULL,
  root = TRUE,
  timestamp = NULL,
  version = NULL,
  stop_layer = NULL,
  integer64 = FALSE,
  fast_root = TRUE,
  method = c("auto", "cloudvolume", "spine"),
  ...
)

Arguments

xyz	One or more xyz locations as an Nx3 matrix or in any form compatible with xyzmatrix including neuron or mesh3d surface objects.
rawcoords	whether the input values are raw voxel indices or in nm
voxdims	voxel dimensions in nm used to convert raw coordinates.
cloudvolume.url	URL for CloudVolume to fetch segmentation image data. The default value of NULL chooses the flywire production segmentation dataset.
root	Whether to return the root id of the whole segment rather than the supervoxel id.
timestamp	An optional timestamp as a string or POSIXct, interpreted as UTC when no timezone is specified.
version	An optional CAVE materialisation version number. See details and examples.
stop_layer	Which layer of the chunked graph to stop at. The default NULL is equivalent to layer 1 or the full root id. Coarser layer 2 IDs can be a useful intermediate for some operations.
integer64	Whether to return ids as integer64 type (more compact but a little fragile) rather than character (default FALSE).
fast_root	Whether to use a fast but two-step look-up procedure when finding roots. This is strongly recommended and the alternative approach has only been retained for validation purposes.
method	Whether to use the spine transform-service API or cloudvolume for lookup. "auto" is presently a synonym for "spine".
...	additional arguments passed to pbapply when looking up multiple positions.

Details

root ids define a whole neuron or segmented object. supervoxel ids correspond to a small group of voxels that it is assumed must all belong to the same object. supervoxel ids do not change for a given a segmentation, whereas root ids change every time a neuron is edited. The most stable way to refer to a FlyWire neuron is to choose a nice safe location on the arbour (I recommend a major branch point) and then store the location and supervoxel id. You can rapidly map the supervoxel id to the current root id using flywire_rootid.

As of November 2020, the default approach to look up supervoxel ids for a 3D point is using the spine transform-service API. This is order 100x faster than mapping via cloudvolume (since that must make a single web request for every point) and Eric Perlman has optimised the layout of the underlying data for rapid mapping.

Note when using the slower cloudvolume method (which may still be required for volumes for which a fast lookup via spine is not available) that finding the supervoxel for a given XYZ location is order 3x faster than finding the root id for the agglomeration of all of the super voxels in a given object. Perhaps less intuitively, if you want to look up many root ids, it is actually quicker to do flywire_xyz2id(,root=F) followed by flywire_rootid since that function can look up many root ids in a single call to the ChunkedGraph server. This is what happens when setting fast_root=TRUE, the default.

Value

A character vector of segment ids, NA when lookup fails.

See Also

Other flywire-ids: flywire_islatest(), flywire_last_modified(), flywire_latestid(), flywire_leaves(), flywire_rootid(), flywire_updateids()

Examples

## Not run: 
# example based on defining some sample points from real neurons
# sample dataset from FAFB catmaid
n=elmr::dense_core_neurons[[1]]
set.seed(42)
ss=sample(nvertices(n), size=10)
# first convert FAF14 points to FlyWire coordinate space
nx=xform_brain(elmr::dense_core_neurons[[1]], ref="FlyWire", sample="FAFB14")
pts=xyzmatrix(nx)[ss,]

## End(Not run)

# for simplicity just define those same sample points directly
pts = matrix(
c(428910.52, 110629.64, 174800, 410201.86, 110419.1, 180400,
  337136, 129926.28, 189280, 349981.85, 136041.53, 199280, 398361.74,
  110731.26, 182640, 382789.26, 118987.04, 189920, 358033.92, 171592.92,
  143960, 360990.48, 140277.42, 201400, 376258.06, 125201.51, 194400,
  331370.31, 128338.58, 181760),
ncol = 3, byrow = TRUE,
dimnames = list(NULL, c("X", "Y", "Z"))
)

#' # Fast and simple appoach to find ids in one (user-facing) step
flywire_xyz2id(pts)


## illustrate what's happening under the hood

# find the ids for the selected xyz locations
# NB fast_root=FALSE was the default behaviour until Nov 2020
flywire_xyz2id(pts, fast_root=FALSE, method="cloudvolume")

# we can also find the supervoxels - much faster
svids=flywire_xyz2id(pts, root=FALSE)

# now look up the root ids - very fast with method="cloudvolume", the default
flywire_rootid(svids)

natverse/fafbseg documentation built on Nov. 11, 2024, 9:50 p.m.