ms_forcescaling: ms_force_scaling

In nkdailingyun/mineCETSA: Processing and Visualization of Proteome-wide MS-CETSA data

Description

Function to apply forced systematic scaling to CETSA melt curve dataset, i.e., to force scale the median of dataset to be same as the median of a subset of dataset (reference dataset)

Usage

ms_forcescaling(
  data,
  refcondition = NULL,
  nread = 10,
  reordertemp = FALSE,
  writefactortofile = TRUE,
  bottomlabel = "Temperature",
  filename = "CETSA_normalization_factors.txt"
)

Arguments

data	dataset to be scaled
refcondition	condition names to specify the reference dataset
nread	number of reading channels or sample treatements
reordertemp	whether to check and re-arrange the treatment temperatures in ascending order, using the readings from lowest temperature group as the reference to derive ratios, default set to FALSE since we typically label the CETSA melt curve samples from low to high temperatures in the TMT10
writefactortofile	whether to save a copy of scaling factors
bottomlabel	textual label at the bottom of the plot
filename	name for the file

Value

a dataframe

Examples

## Not run: 
LY_forcescaled <- ms_forcescaling(LY_cleaned)

## End(Not run)

nkdailingyun/mineCETSA documentation built on Feb. 27, 2021, 8:26 p.m.