TrackCellsDataset: Sample Stack of Fluorescent Cells

In ocbe-uio/CellMigRation: Track Cells, Analyze Cell Trajectories and Compute Migration Statistics

Description

Sample Stack of Fluorescent Cells to be used for computing cell tracks and stats

Usage

data(TrackCellsDataset)

Format

a trackedCells object including 10 stacks

Details

This stack of Fluorescent Cell images was obtained by processing TIFF files via cellmigRation. Imaging experiments were performed as described by Ghannoum S et al (paper in preparation). Briefly, triple negative breast cancer BT549 cells were cultured in RPMI supplemented with 10 and 1 NucLight green lentivirus (Essen BioScience), and then sorted by fluorescence-activated cell sorting (FACS). GFP-positive cells were seeded at a 1:3 ratio with untransduced BT549 cells in 96-well image-lock plates (EssenBio) at a density of 1000 total cells per well. Cells were scanned at ten-minute intervals over 24h using an Incucyte S3 Live-Cell microscope (EssenBio) at 10x magnification and a Basler Ace 1920-155um camera with CMOS sensor. A small selection of cells and image stacks is included in this dataset.

Examples

data(TrackCellsDataset)

ocbe-uio/CellMigRation documentation built on Dec. 26, 2021, 10:19 p.m.