Description Usage Arguments Value
Each column is divided by the 75 The calculated quantile is then scaled by the median across cells to keep the absolute level of expression relatively consistent. Spike-ins should be excluded from the calculation of total expression in order to correct for total cell RNA content, therefore they should be specified to exclude them from downstream analysis
1 |
expr_mat |
Input matrix with expression values for genes in the form (Genes x Samples) |
spikes |
A vector of genes that were spike-ins in the original experiment |
A matrix with columns upper quartile normalized.
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