uq_scale: Upper Quartile Normalization

Description Usage Arguments Value

Description

Each column is divided by the 75 The calculated quantile is then scaled by the median across cells to keep the absolute level of expression relatively consistent. Spike-ins should be excluded from the calculation of total expression in order to correct for total cell RNA content, therefore they should be specified to exclude them from downstream analysis

Usage

1
uq_scale(expr_mat, spikes = NULL)

Arguments

expr_mat

Input matrix with expression values for genes in the form (Genes x Samples)

spikes

A vector of genes that were spike-ins in the original experiment

Value

A matrix with columns upper quartile normalized.


ssarda/genomeutils documentation built on May 30, 2019, 8:42 a.m.