Description Usage Arguments Examples
Calculate the vector volume for In-Fusion reaction
1 2 3 4 5 6 7 8 9 | calc_InFusion_vector_volume(
seq_i,
seq_v,
conc_i,
conc_v,
uL_i,
ratio_i = 2,
ratio_v = 1
)
|
seq_i |
DNA sequence of insertion |
seq_v |
DNA sequence of vector |
conc_i |
DNA concentration (ng/uL) of purified insertion |
conc_v |
DNA concentration (ng/uL) of purified vector |
uL_i |
Supplied volume (uL) of purified insertion |
ratio_i |
Molecular ratio of insertion |
ratio_v |
Molecular ratio of vector |
1 2 3 4 5 6 | calc_InFusion_vector_volume(
seq_i = dstr_rand_seq(1, 1000, seed = 1),
seq_v = dstr_rand_seq(1, 5000, seed = 1),
conc_i = 5, conc_v = 30,
uL_i = 3
)
|
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