calc_InFusion_vector_volume: Calculate the vector volume for In-Fusion reaction

Description Usage Arguments Examples

View source: R/infusion.R

Description

Calculate the vector volume for In-Fusion reaction

Usage

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calc_InFusion_vector_volume(
  seq_i,
  seq_v,
  conc_i,
  conc_v,
  uL_i,
  ratio_i = 2,
  ratio_v = 1
)

Arguments

seq_i

DNA sequence of insertion

seq_v

DNA sequence of vector

conc_i

DNA concentration (ng/uL) of purified insertion

conc_v

DNA concentration (ng/uL) of purified vector

uL_i

Supplied volume (uL) of purified insertion

ratio_i

Molecular ratio of insertion

ratio_v

Molecular ratio of vector

Examples

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calc_InFusion_vector_volume(
  seq_i = dstr_rand_seq(1, 1000, seed = 1),
  seq_v = dstr_rand_seq(1, 5000, seed = 1),
  conc_i = 5, conc_v = 30,
  uL_i = 3
)

t-arae/bstringr documentation built on March 18, 2021, 3:08 a.m.