getDeadLocations: Find dead cultures in SGA plates (1536 format), and report...
In qfa: Tools for Quantitative Fitness Analysis (QFA) of Arrayed Microbial Cultures Growing on Solid Agar Surfaces
Description
Usage
Arguments
Value
Description
Given Colonyzer quantifications of SGA plates in 1536 format, locate dead cultures and report the 384-format locations of those cultures for stripping.
Usage
1
getDeadLocations(SGAFile,SGAExpt,CutoffFrac=0.0025)
Arguments
SGAFile
The Colonyzer output for the final SGA plates. These are 1536 format plates, and the Colonyzer file should be a .dat tab delimited text file.
SGAExpt
Experiment description file for the SGA, linking barcode with plate number.
CutoffFrac
Optional argument for specifying the minimum value for Growth (normalised IOD) corresponding to detection of cells on 1536 plate.
Value
A data frame containing columns ROW384, COLUMN384, REP384 (repeat or quadrant identifier) and PLATE which will be useful for stripping.
qfa documentation built on Feb. 22, 2020, 3:01 a.m.
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Description Usage Arguments Value
Description
Given Colonyzer quantifications of SGA plates in 1536 format, locate dead cultures and report the 384-format locations of those cultures for stripping.
Usage
1 | getDeadLocations(SGAFile,SGAExpt,CutoffFrac=0.0025)
|
Arguments
SGAFile |
The Colonyzer output for the final SGA plates. These are 1536 format plates, and the Colonyzer file should be a .dat tab delimited text file. |
SGAExpt |
Experiment description file for the SGA, linking barcode with plate number. |
CutoffFrac |
Optional argument for specifying the minimum value for Growth (normalised IOD) corresponding to detection of cells on 1536 plate. |
Value
A data frame containing columns ROW384, COLUMN384, REP384 (repeat or quadrant identifier) and PLATE which will be useful for stripping.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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