UserMetadata-class: UserMetadata S4 class
In BgeeCall: Automatic RNA-Seq present/absent gene expression calls generation

Description Slots

An S4 class containing all metadata that have to be provided by the user It is mandatory to edit 'species_id', 'rnaseq_lib_path', 'transcriptome_path', 'annotation_name', 'annotation_object' and potentialy 'run_ids' before using the package.

species_id: The NCBI Taxon Id of the species
run_ids: A vector of charater. Has to be provided only if a subset of runs present in UserMetadata@rnaseq_lib_path has to be run. If empty, all fastq files present in the rnaseq_lib_path will be considered as technical replicates and merged to run one transcript expression estimation analyse.
reads_size: The size of the reads. If smaller than 'KallistoMetadata@read_size_kmer_threshold', an index with a kmer size of 15 bp will be used.
rnaseq_lib_path: Path to the directory of the RNA-Seq library that contains fastq files. The extension of the fastq files name must be .fq, .fastq, .fq.gz, or .fastq.gz
transcriptome_name: Name of the transcriptome used to generate arborescence of output repositories.
transcriptome_object: Object containing transcriptome
annotation_name: Name of the annotation used to generate arborescence of output repositories.
annotation_object: Object containing annotations from GTF or GFF file
working_path: Working directory. By default the working directory is defined with the 'getwd()' function.
simple_arborescence: logical allowing to create a simple arborescence of directory. If 'TRUE' (default), all results will be on the same directory (working_path/intergenic_release/all_results/libraryId). Use 'FALSE' if you plan to generate expression calls for the same library using different transcriptomes or gene annotations, otherwise you will overwrite previous results. When 'FALSE' the path to result folder looks like : working_path/intergenic_release/speciesId/kallisto/transcriptome_name/annotation_name/libraryId
output_dir: (optional) Allows to manually define your output directory. By default the path to output directory is created automatically from the working_path (working_path/intergenic_release/all_results/libraryId/).
verbose: logical allowing to use the verbose mode. TRUE by default.
custom_intergenic_path: path to a local version of reference intergenic fasta file. If NULL (by default) the reference intergenic fasta file will be downloaded. If not NULL BgeeCall will merge this local reference intergenic file with the transcriptome. Except if you generated your own intergenic regions always keep it NULL.