replaceLetterAt first makes a copy of a sequence (or set of
sequences) and then replaces some of the original letters by new
letters at the specified locations.
.inplaceReplaceLetterAt is the IN PLACE version of
replaceLetterAt: it will modify the original
sequence in place i.e. without copying it first.
Note that in place modification of a sequence is fundamentally
dangerous because it alters all objects defined in your session
that make reference to the modified sequence.
.inplaceReplaceLetterAt, unless you know what
you are doing!
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A DNAString or rectangular DNAStringSet object.
The locations where the replacements must occur.
The new letters.
What to do if the new letter is not "extending" the old letter?
The new letter "extends" the old letter if both are IUPAC letters
and the new letter is as specific or less specific than the old
one (e.g. M extends A, Y extends Y, but Y doesn't extend S).
Possible values are
Note that the gap (
Also note that
.inplaceReplaceLetterAt semantic is equivalent to calling
It is used by the
in the BSgenome package, as part of the "lazy sequence loading"
mechanism, for altering the original sequences of a
BSgenome object at "sequence-load time".
This alteration consists in injecting the IUPAC ambiguity letters
representing the SNPs into the just loaded sequence, which is the
only time where in place modification of the external data of
an XString object is safe.
A DNAString or DNAStringSet object of the same shape
(i.e. length and width) as the orignal object
replaceAt function for extracting or replacing
arbitrary subsequences from/in a sequence or set of sequences.
IUPAC_CODE_MAP for the mapping between IUPAC
nucleotide ambiguity codes and their meaning.
The DNAString and DNAStringSet class.
injectSNPs function and the
BSgenome class in the BSgenome package.
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## Replace letters of a DNAString object: replaceLetterAt(DNAString("AAMAA"), c(5, 1, 3, 1), "TYNC") replaceLetterAt(DNAString("AAMAA"), c(5, 1, 3, 1), "TYNC", if.not.extending="merge") ## Replace letters of a DNAStringSet object (sorry for the totally ## artificial example with absolutely no biological meaning): library(drosophila2probe) probes <- DNAStringSet(drosophila2probe) at <- matrix(c(TRUE, TRUE, FALSE, FALSE, FALSE, TRUE, FALSE, FALSE), nrow=length(probes), ncol=width(probes), byrow=TRUE) letter_subject <- DNAString(paste(rep.int("-", width(probes)), collapse="")) letter <- as(Views(letter_subject, start=1, end=rowSums(at)), "XStringSet") replaceLetterAt(probes, at, letter)