Description Usage Arguments Value Author(s)
This function reads a raw Fluidigm Biomark data file or set of files and constructs a SingleCellAssay (or FluigidmAssay) object. This was written c. 2011 and has not been tested lately. The Biomark format may have changed.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 | read.fluidigm(
files = NULL,
metadata = NULL,
header.size = 2,
skip = 8,
cycle.threshold = 40,
metadataColClasses = NULL,
meta.key = NULL,
idvars = NULL,
splitby = NULL,
unique.well.id = "Chamber.ID",
raw = TRUE,
assay = NULL,
geneid = "Assay.Name",
sample = NULL,
well = "Well",
measurement = "X40.Ct",
measurement.processed = "Ct",
ncells = "SampleRConc"
)
|
files |
A |
metadata |
A |
header.size |
A |
skip |
|
cycle.threshold |
The maximum number of PCR cycles performed (default 40) |
metadataColClasses |
Optional |
meta.key |
Optional |
idvars |
Optional |
splitby |
Optional |
unique.well.id |
The column that uniquely identifies a sample well in the data. Default is "Chamber.ID". |
raw |
|
assay |
|
geneid |
|
sample |
|
well |
|
measurement |
|
measurement.processed |
|
ncells |
The column with the number of cells in this well. |
list of SingleCellAssay
holding the data.
Greg Finak
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