annotationBlocksCounts: Counts the number of sequencing reads within supplied genomic...

In Repitools: Epigenomic tools

Description

Counts reads inside blocks.

Usage

  ## S4 method for signature 'ANY,data.frame'
annotationBlocksCounts(x, anno, ...)
  ## S4 method for signature 'character,GRanges'
annotationBlocksCounts(x, anno, ...)
  ## S4 method for signature 'GRanges,GRanges'
annotationBlocksCounts(x, anno, seq.len = NULL, verbose = TRUE)
  ## S4 method for signature 'GRangesList,GRanges'
annotationBlocksCounts(x, anno, ...)

Arguments

x	A character vector of BAM paths, a GRangesList, or GRanges object.
anno	A set of genomic features to make windows around a reference point of theirs. Either a data.frame with (at least) colums chr, start, and end, or a GRanges object.
seq.len	If sequencing reads need to be extended, the fragment size to be used. Default: NULL (no extension).
verbose	Whether to print progress. Default: TRUE.
...	Parameters described above, that are not used in the top-level error-checking stage, but are passed further into a private function that uses them in its processing.

Value

A matrix of counts is returned, one column per sample and one row per row of genomic features supplied.

Author(s)

Aaron Statham

See Also

annotationCounts, genomeBlocks

Examples

  require(GenomicRanges)
  reads <- GRanges(seqnames = rep("chr1", 5),
                   IRanges(c(3309, 4756, 4801, 4804, 5392), width = 36),
                   strand = c('+', '-', '-', '+', '+'))
  genes <- GRanges("chr1", IRanges(5000, 7000), strand = '+')
  annotationBlocksCounts(reads, genes, 300)

Repitools documentation built on Nov. 8, 2020, 7:52 p.m.