Description Usage Arguments Details Value Author(s) See Also Examples

`srdistance`

calculates the edit distance from each read in
`pattern`

to each read in `subject`

. The underlying
algorithm `pairwiseAlignment`

is only efficient when both
reads are short, and when the number of `subject`

reads is small.

1 | ```
srdistance(pattern, subject, ...)
``` |

`pattern` |
An object of class |

`subject` |
A short |

`...` |
additional arguments, unused. |

The underlying algorithm performs pairwise alignment from each read in
`pattern`

to each sequence in `subject`

. The return value is
a list of numeric vectors of distances, one list element for each
sequence in `subject`

. The vector in each list element contains
for each read in `pattern`

the edit distance from the read to the
corresponding subject. The weight matrix and gap penalties used to
calculate the distance are structured to weight base substitutions and
single base insert/deletions equally. Edit distance between known and
ambiguous (e.g., N) nucleotides, or between ambiguous nucleotides, are
weighted as though each possible nucleotide in the ambiguity were
equally likely.

A list of length equal to that of `subject`

. Each element is a
numeric vector equal to the length of `pattern`

, with values
corresponding to the minimum distance between between the
corresponding pattern and subject sequences.

Martin Morgan <[email protected]>

1 2 3 4 5 6 7 8 | ```
sp <- SolexaPath(system.file("extdata", package="ShortRead"))
aln <- readAligned(sp, "s_2_export.txt")
polyA <- polyn("A", 35)
polyT <- polyn("T", 35)
d1 <- srdistance(clean(sread(aln)), polyA)
d2 <- srdistance(sread(aln), polyA)
d3 <- srdistance(sread(aln), c(polyA, polyT))
``` |

ShortRead documentation built on Feb. 23, 2018, 2 a.m.

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