Pick based primers from designed primers for every target based on Primer3 penalty score or
off-target priming estimated with
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The number of top primers to pick (default: 1, which returns the best primer).
Attribute by which primers should be picked. Can be either
by is set to
off_targets top primers are picked based on the lowest number of
exonic, intronic and intergenic off-targets (in that priority).
object containing the picked primers.
TsIO: Pick best primers in a
TsIOList: Pick best primers per target in a
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# chr11 primers examples data("chr11_primers") # pick the best primer per gene based on the fewest exonic, intronic and intergenic off-targets # (in that order) best_primers <- pickPrimers(chr11_primers, by = "off_targets") tapseq_primers(best_primers) # pick the best two primers per gene based on the lowest penalty score computed by Primer3 best_primers <- pickPrimers(chr11_primers, n = 2, by = "penalty") tapseq_primers(best_primers)
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