expressionQCPipeline: Flexible bead-level QC pipeline

In beadarray: Quality assessment and low-level analysis for Illumina BeadArray data

Description

Function to produce various QC plots and HTML summary pages for bead-level data.

Usage

expressionQCPipeline(BLData, transFun = logGreenChannelTransform, qcDir = "QC", plotType = ".jpeg", horizontal = TRUE, controlProfile = NULL, overWrite=FALSE,nSegments=9,outlierFun=illuminaOutlierMethod,tagsToDetect = list(housekeeping = "housekeeping", Biotin = "biotin", Hybridisation = "cy3_hyb"),zlim=c(5,7),positiveControlTags = c("housekeeping", "biotin"), hybridisationTags =  c("cy3_hyb"), negativeTag= "negative", boxplotFun = logGreenChannelTransform, imageplotFun = logGreenChannelTransform)

Arguments

BLData	a beadLevelData object
transFun	what transformation function to apply
qcDir	a directory to write output to
plotType	desired file extension for plots (jpeg or png)
horizontal	if TRUE imageplots and outlier plots are produced with longest edge on x axis
controlProfile	a data frame defining all control types. not required if annotation information is stored in the bead-level object
overWrite	if FALSE any plots that exist in the directory will not be recreated
nSegments	how many segments each section is divided into
outlierFun	a function to removed outliers
tagsToDetect	which control types to used in the detection metrics
zlim	the range of the imageplots
boxplotFun	what transformation function to be used in boxplots
imageplotFun	what transformation function to be used for imageplots
positiveControlTags	character strings defining which positive controls to plot
hybridisationTags	additional control types to be plotted
negativeTag	character string to identify which control type in the control profile corresponds to negative controls

Details

This function is a convient way of automatically generating QC plots for each section within a beadLevelData object. The following plots are produced for each section. i) scatter plots of all bead observation of the positive controls. See poscontPlot. ii) Further scatter plots of other controls of interest using poscontPlot. iii) imageplot (imageplot) of section data after applying transformation function iv) plot of outlier locations using specified outlier function. A HTML page displaying all the plots is produced.

After plots have been produced for each section, makeQCTable is run to make a table of mean and standard deviations for the defined control types, followed by the results of calculateOutlierStats and controlProbeDetection for each section and written to a HTML page in the requested directory.

The function should be able to run automatically for expression data that has its annotation stored using setAnnotation or using readIllumina. Otherwise the controlProfile data frame can be used to define the control types on the array and their associated ArrayAddressIDs. Similarly, the function assumes single-channel data but a transformation function can be passed.

Author(s)

Mark Dunning

See Also

poscontPlot imageplot outlierplot controlProbeDetection

Examples

if(require(beadarrayExampleData)){

## Not run: 

data(exampleBLData)

expressionQCPipeline(exampleBLData, horizontal=T)


## End(Not run)

}

beadarray documentation built on Nov. 8, 2020, 4:51 p.m.