FlowStandards: An S4 class to represent internal standard details for...
In flowPloidy: Analyze flow cytometer data to determine sample ploidy

Description Usage Arguments Value Slots Examples

The sizes slot is set in FlowHist or batchFlowHist. The other values are updates through interaction with the browseFlowHist GUI.

stdSizes(std)

stdSelected(std)

stdPeak(std)

std

a FlowStandards object

stdSizes, stdSelected and stdPeak return the corresponding slot values

sizes: numeric, the size (in pg) of the internal size standard. Can be a vector of multiple values, if the sample is part of a set that included different standards for different samples.
selected: numeric, the size (in pg) of the internal size standard actually used for this sample. Must be one of the values in the sizes slot.
peak: character, "A" or "B", indicating which of the histogram peaks is the size standard.

library(flowPloidyData) 
fh1 <- FlowHist(file = flowPloidyFiles()[1], channel = "FL3.INT.LIN",
                standards = c(1.96, 5.43))
fhStandards(fh1)  ## display standards included in this object
stdSizes(fhStandards(fh1))  ## list standard sizes