Brief Information of removed Cell-events by immunoClust Cell-event Clustering

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Description

Gives information about the amount of overexposed cell-event observetion in a FCS-file.

Usage

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removed.above(fcs, parameters=NULL, N=NULL, max.count=10, max=NULL)
removed.below(fcs, parameters=NULL, N=NULL, min.count=10, min=NULL)

Arguments

fcs

An object of class flowFrame. Rows correspond to observations and columns correspond to measured parameters.

parameters

A character vector specifying the parameters (columns) to be included in clustering. When it is left unspecified, all the parameters will be used.

N

Maximum number of observations used for clustering. When unspecified or higher than the number of observations (i.e. rows) in dat, all observations are used for clustering, otherwise only the first N observations.

max.count

An integer specifying the threshold count for filtering data points from above. The default is 10, meaning that if 10 or more data points are larger than or equal to max, they will be excluded from the analysis. If max is NULL, then the maximum value of each parameter will be used. To suppress filtering, it is set to -1.

max

The upper limit set for data filtering. Note that it is a vector of length equal to the number of parameters (columns), implying that a different value can be set for each parameter.

min.count

analoguous to max.count.

min

analoguous to min.

Value

A table with two rows containing the number of events above max in each parameter and above in only this parameter. The two last columns give the sum and percentage of all events above max in any parameter.

Author(s)

Till Sörensen till-antoni.soerensen@charite.de

Examples

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