calc.delta.score: Calculate Delta Score from Ion Score

In isobar: Analysis and quantitation of isobarically tagged MSMS proteomics data

Description

Calculates delta score from raw search engine score by substracting the best matching hit with the second best matching. data needs to have not only the best hit per spectrum, but multiple, to be able to calculate the delta score. filterSpectraDeltaScore calls calc.delta.score and filters spectra below a minum delta score.

Usage

calc.delta.score(my.data)
filterSpectraDeltaScore(my.data, min.delta.score=10, do.remove=FALSE)

Arguments

my.data	IBSpectra data frame.
min.delta.score	Minimum delta score.
do.remove	If TRUE, spectra below the min.prob threshold are not just set as 'use.for.quant=FALSE' but removed.

Value

Returns data with additional column 'delta.score'.

Author(s)

Florian P. Breitwieser

isobar documentation built on Nov. 8, 2020, 7:48 p.m.