Nothing
GetComponentsToExportInHTMLreport <- function(parameterizationIndex=NULL)
{
Try(NumParameterizations <- get("NumParameterizations",envir=limmaGUIenvironment))
Try(LinearModelComputed <- get("LinearModelComputed", envir=limmaGUIenvironment))
Try(ndups <- get("ndups",envir=limmaGUIenvironment))
Try(ttHTMLreportDialog<-tktoplevel(.limmaGUIglobals$ttMain))
Try(tkwm.deiconify(ttHTMLreportDialog))
Try(tkgrab.set(ttHTMLreportDialog))
Try(tkfocus(ttHTMLreportDialog))
Try(tkwm.title(ttHTMLreportDialog,"HTML Report"))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" ")))
Try(TargetsTcl <- tclVar("1"))
Try(SpotTypesTcl <- tclVar("1"))
Try(LayoutTcl <- tclVar("1"))
Try(BackgroundCorrectionTcl <- tclVar("1"))
Try(SpotWeightingTcl <- tclVar("1"))
Try(RawMATcl <- tclVar("1"))
Try(RawPrintTipGroupTcl <- tclVar("1"))
Try(ScaleBoxPlotTcl <- tclVar("1"))
Try(if (NumParameterizations>0)
Try(SpotTypesInLinearModelTcl <- tclVar("1"))
else
Try(SpotTypesInLinearModelTcl <- tclVar("0")))
Try(if (NumParameterizations>0)
Try(NormalizationInLinearModelTcl <- tclVar("1"))
else
Try(NormalizationInLinearModelTcl <- tclVar("0")))
Try(if (NumParameterizations>0)
Try(DesignMatrixTcl <- tclVar("1"))
else
Try(DesignMatrixTcl <- tclVar("0")))
Try(if (NumParameterizations>0 && ndups>1)
Try(DupCorTcl <- tclVar("1"))
else
Try(DupCorTcl <- tclVar("0")))
Try(if (NumParameterizations>0)
Try(Top50ToptablesTcl <- tclVar("1"))
else
Try(Top50ToptablesTcl <- tclVar("0")))
Try(CompleteToptablesTcl <- tclVar("0"))
Try(if (NumParameterizations>0)
Try(AvgMAPlotTcl <- tclVar("1"))
else
Try(AvgMAPlotTcl <- tclVar("0")))
Try(tStatisticBoxPlotsTcl <- tclVar("0"))
Try(TargetsCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=TargetsTcl))
Try(SpotTypesCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=SpotTypesTcl))
Try(LayoutCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=LayoutTcl))
Try(BackgroundCorrectionCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=BackgroundCorrectionTcl))
Try(SpotWeightingCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=SpotWeightingTcl))
Try(RawMACheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=RawMATcl))
Try(RawPrintTipGroupCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=RawPrintTipGroupTcl))
Try(ScaleBoxPlotCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=ScaleBoxPlotTcl))
Try(SpotTypesInLinearModelCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=SpotTypesInLinearModelTcl))
Try(NormalizationInLinearModelCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=NormalizationInLinearModelTcl))
Try(DesignMatrixCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=DesignMatrixTcl))
Try(DupCorCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=DupCorTcl))
Try(Top50ToptablesCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=Top50ToptablesTcl))
Try(CompleteToptablesCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=CompleteToptablesTcl))
Try(AvgMAPlotCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=AvgMAPlotTcl))
Try(tStatisticBoxPlotsCheckbox <- tkcheckbutton(ttHTMLreportDialog,variable=tStatisticBoxPlotsTcl))
Try(lbl2 <- tklabel(ttHTMLreportDialog,text="Components to be Included in the HTML Report",font=.limmaGUIglobals$limmaGUIfont2))
tkgrid(tklabel(ttHTMLreportDialog,text=" "),lbl2)
Try(tkgrid.configure(lbl2,columnspan=3,sticky="w"))
tkgrid(tklabel(ttHTMLreportDialog,text=" "))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="RNA Targets",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),TargetsCheckbox,currentLabel))
Try(tkgrid.configure(TargetsCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Spot Types",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),SpotTypesCheckbox,currentLabel))
Try(tkgrid.configure(SpotTypesCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Layout",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),LayoutCheckbox,currentLabel))
Try(tkgrid.configure(LayoutCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Background Correction Method",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),BackgroundCorrectionCheckbox,currentLabel))
Try(tkgrid.configure(BackgroundCorrectionCheckbox,sticky="e"));Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Spot Quality Weighting",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),SpotWeightingCheckbox,currentLabel))
Try(tkgrid.configure(SpotWeightingCheckbox,sticky="e"));Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Color-Coded Raw M A Plots",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),RawMACheckbox,currentLabel))
Try(tkgrid.configure(RawMACheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Print-Tip Group Loess Raw M A Plots",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),RawPrintTipGroupCheckbox,currentLabel))
Try(tkgrid.configure(RawPrintTipGroupCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Within-Array Normalized M Box Plots",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),ScaleBoxPlotCheckbox,currentLabel))
Try(tkgrid.configure(ScaleBoxPlotCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Spot Types Included In Linear Model",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),SpotTypesInLinearModelCheckbox,currentLabel))
Try(tkgrid.configure(SpotTypesInLinearModelCheckbox,sticky="e"));Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Normalization Used In Linear Model",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),NormalizationInLinearModelCheckbox,currentLabel))
Try(tkgrid.configure(NormalizationInLinearModelCheckbox,sticky="e"));Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Design Matrix (Parameterization)",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),DesignMatrixCheckbox,currentLabel))
Try(tkgrid.configure(DesignMatrixCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Duplicate Correlation",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),DupCorCheckbox,currentLabel))
Try(tkgrid.configure(DupCorCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Top 50 DE Genes",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),Top50ToptablesCheckbox,currentLabel))
Try(tkgrid.configure(Top50ToptablesCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="Complete Lists of DE-Ranked Genes",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),CompleteToptablesCheckbox,currentLabel))
Try(tkgrid.configure(CompleteToptablesCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="M A Plots (with fitted M values)",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),AvgMAPlotCheckbox,currentLabel))
Try(tkgrid.configure(AvgMAPlotCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
Try(currentLabel <- tklabel(ttHTMLreportDialog,text="t Statistic Box Plots",font=.limmaGUIglobals$limmaGUIfont2))
Try(tkgrid(tklabel(ttHTMLreportDialog,text=" "),tStatisticBoxPlotsCheckbox,currentLabel))
Try(tkgrid.configure(tStatisticBoxPlotsCheckbox,sticky="e")); Try(tkgrid.configure(currentLabel,sticky="w",columnspan=2))
# Need to test whether arrays have been loaded and whether a linear model has been fit.
if (NumParameterizations==0 || LinearModelComputed[parameterizationIndex]==FALSE)
{
Try(tkconfigure(SpotTypesInLinearModelCheckbox,state="disabled"))
Try(tkconfigure(NormalizationInLinearModelCheckbox,state="disabled"))
Try(tkconfigure(DesignMatrixCheckbox,state="disabled"))
Try(tkconfigure(DupCorCheckbox,state="disabled"))
Try(tkconfigure(Top50ToptablesCheckbox,state="disabled"))
Try(tkconfigure(CompleteToptablesCheckbox,state="disabled"))
Try(tkconfigure(tStatisticBoxPlotsCheckbox,state="disabled"))
Try(tkconfigure(AvgMAPlotCheckbox,state="disabled"))
}
if (ndups<=1)
Try(tkconfigure(DupCorCheckbox,state="disabled"))
tkgrid(tklabel(ttHTMLreportDialog,text=" "))
tkgrid(tklabel(ttHTMLreportDialog,text=" "))
ReturnVal <- list()
onOK <- function()
{
if (tclvalue(TargetsTcl)=="1") ReturnVal[[1]] <- TRUE else ReturnVal[[1]] <- FALSE; attributes(ReturnVal)$names[1] <- "Targets"
if (tclvalue(SpotTypesTcl)=="1") ReturnVal[[2]] <- TRUE else ReturnVal[[2]] <- FALSE; attributes(ReturnVal)$names[2] <- "SpotTypes"
if (tclvalue(LayoutTcl)=="1") ReturnVal[[3]] <- TRUE else ReturnVal[[3]] <- FALSE; attributes(ReturnVal)$names[3] <- "Layout"
if (tclvalue(BackgroundCorrectionTcl)=="1") ReturnVal[[4]] <- TRUE else ReturnVal[[4]] <- FALSE; attributes(ReturnVal)$names[4] <- "BackgroundCorrection"
if (tclvalue(SpotWeightingTcl)=="1") ReturnVal[[5]] <- TRUE else ReturnVal[[5]] <- FALSE; attributes(ReturnVal)$names[5] <- "SpotWeighting"
if (tclvalue(RawMATcl)=="1") ReturnVal[[6]] <- TRUE else ReturnVal[[6]] <- FALSE; attributes(ReturnVal)$names[6] <- "RawMA"
if (tclvalue(RawPrintTipGroupTcl)=="1") ReturnVal[[7]] <- TRUE else ReturnVal[[7]] <- FALSE; attributes(ReturnVal)$names[7] <- "RawPrintTipGroup"
if (tclvalue(ScaleBoxPlotTcl)=="1") ReturnVal[[8]] <- TRUE else ReturnVal[[8]] <- FALSE; attributes(ReturnVal)$names[8] <- "ScaleBoxPlot"
if (tclvalue(SpotTypesInLinearModelTcl)=="1") ReturnVal[[9]] <- TRUE else ReturnVal[[9]] <- FALSE; attributes(ReturnVal)$names[9] <- "SpotTypesInLinearModel"
if (tclvalue(NormalizationInLinearModelTcl)=="1") ReturnVal[[10]] <- TRUE else ReturnVal[[10]] <- FALSE; attributes(ReturnVal)$names[10] <- "NormalizationInLinearModel"
if (tclvalue(DesignMatrixTcl)=="1") ReturnVal[[11]] <- TRUE else ReturnVal[[11]] <- FALSE; attributes(ReturnVal)$names[11] <- "DesignMatrix"
if (tclvalue(DupCorTcl)=="1") ReturnVal[[12]] <- TRUE else ReturnVal[[12]] <- FALSE; attributes(ReturnVal)$names[12] <- "DupCor"
if (tclvalue(Top50ToptablesTcl)=="1") ReturnVal[[13]] <- TRUE else ReturnVal[[13]] <- FALSE; attributes(ReturnVal)$names[13] <- "Top50Toptables"
if (tclvalue(CompleteToptablesTcl)=="1") ReturnVal[[14]] <- TRUE else ReturnVal[[14]] <- FALSE; attributes(ReturnVal)$names[14] <- "CompleteToptables"
if (tclvalue(AvgMAPlotTcl)=="1") ReturnVal[[15]] <- TRUE else ReturnVal[[15]] <- FALSE; attributes(ReturnVal)$names[15] <- "AvgMAPlot"
if (tclvalue(tStatisticBoxPlotsTcl)=="1") ReturnVal[[16]] <- TRUE else ReturnVal[[16]] <- FALSE; attributes(ReturnVal)$names[16] <- "tStatisticBoxPlots"
Try(tkgrab.release(ttHTMLreportDialog));Try(tkdestroy(ttHTMLreportDialog));Try(tkfocus(.limmaGUIglobals$ttMain))
ReturnVal <<- ReturnVal
}
onCancel <- function() {Try(tkgrab.release(ttHTMLreportDialog));Try(tkdestroy(ttHTMLreportDialog));Try(tkfocus(.limmaGUIglobals$ttMain)); ReturnVal <<- list()}
OK.but <-tkbutton(ttHTMLreportDialog,text=" OK ",command=onOK,font=.limmaGUIglobals$limmaGUIfont2)
Cancel.but <-tkbutton(ttHTMLreportDialog,text=" Cancel ",command=onCancel,font=.limmaGUIglobals$limmaGUIfont2)
tkgrid(tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" "),OK.but,Cancel.but,tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" "))
tkgrid.configure(OK.but, sticky="e")
tkgrid.configure(Cancel.but,sticky="w")
tkgrid(tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" "),
tklabel(ttHTMLreportDialog,text=" "),tklabel(ttHTMLreportDialog,text=" "))
Try(tkfocus(ttHTMLreportDialog))
Try(tkbind(ttHTMLreportDialog, "<Destroy>", function() {Try(tkgrab.release(ttHTMLreportDialog));Try(tkfocus(.limmaGUIglobals$ttMain));}))
Try(tkwait.window(ttHTMLreportDialog))
return (ReturnVal)
}
###########################################################################################################################
# R2HTML Plot Function (Modified to accept a plotFunction argument, rather than using the main R Graphics Device)
"HTMLplotUsingFunction" <- function (Caption = "", File = .HTML.file, GraphRelativeDirectory = ".", GraphAbsoluteDirectory = NULL, GraphFileName = "", GraphSaveAs = "png", GraphBorder = 1, Align = "center", plotFunction = NULL,Width=600,Height=600,PointSize=12,BG="white",res=72,...)
{
if (is.null(GraphAbsoluteDirectory))
GraphAbsoluteDirectory <- getwd()
if (GraphFileName == "") {
nowd <- date()
GraphFileName <- paste("GRAPH_", substring(nowd, 5, 7), substring(nowd, 9, 10), "_", substring(nowd, 12, 13), substring(nowd, 15, 16), substring(nowd, 18, 19), sep = "")
}
GraphFileName <- paste(GraphFileName, ".", GraphSaveAs, sep = "")
# AbsGraphFileName <- paste(GraphRelativeDirectory,.Platform$file.sep,GraphFileName,sep="")
AbsGraphFileName <- file.path(GraphAbsoluteDirectory, GraphFileName)
if (GraphSaveAs=="png")
{
if (is.null(plotFunction))
dev.print(png, file = AbsGraphFileName, width=Width,height=Height,pointsize=PointSize,bg=BG)
else
{
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows" && Sys.info()["sysname"] != "Darwin")
Try(bitmap(file = AbsGraphFileName,bg=BG,res=res))
else
Try(png(filename = AbsGraphFileName, width=Width,height=Height,pointsize=PointSize,bg=BG)))
plotFunction()
dev.off()
}
}
else if (GraphSaveAs=="jpg")
{
if (is.null(plotFunction))
dev.print(jpeg, file = AbsGraphFileName, width=Width,height=Height,pointsize=PointSize,bg=BG)
else
{
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows" && Sys.info()["sysname"] != "Darwin")
Try(bitmap(filename = AbsGraphFileName,bg=BG,res=res,type="jpeg"))
else
Try(jpeg(filename = AbsGraphFileName, width=Width,height=Height,pointsize=PointSize,bg=BG)))
plotFunction()
dev.off()
}
}
else if (GraphSaveAs=="gif")
{
if (is.null(plotFunction))
dev.print(gif, file = AbsGraphFileName, width=Width,height=Height,pointsize=PointSize,bg=BG)
else
{
stop("When passing a plot function to HTMLplot, device must be jpg or png.")
}
}
else stop("GraphSaveAs must be either jpg, png or gif")
cat(paste("<p align=", Align, "><img src='", paste(GraphRelativeDirectory,"/",GraphFileName,sep=""), "' border=", GraphBorder, ">", sep = "", collapse = ""), file = File, append = TRUE, sep = "")
if (Caption != "") {
cat(paste("<br><i>", Caption, "</i>"), file = File, append = TRUE, sep = "")
}
cat("</P>", file = File, append = TRUE, sep = "\n")
try(assign(".HTML.graph", value=TRUE, envir = get("HTMLenv", envir = .GlobalEnv)))
invisible(return())
}
###########################################################################################################################
ExportHTMLreport <- function()
{
# We will use the R2HTML package, but with my own HTMLplot function.
# Will we need xtable or does R2HTML have its own HTMLtable function?
#Require("xtable")
#Require("R2HTML")
Try(limmaDataSetNameText <- get("limmaDataSetNameText",envir=limmaGUIenvironment))
Try(ArraysLoaded <- get("ArraysLoaded", envir=limmaGUIenvironment))
Try(NumParameterizations <- get("NumParameterizations",envir=limmaGUIenvironment))
Try(LinearModelComputed <- get("LinearModelComputed", envir=limmaGUIenvironment))
Try(ParameterizationTreeIndexVec <- get("ParameterizationTreeIndexVec",envir=limmaGUIenvironment))
Try(RG <- get("RG",envir=limmaGUIenvironment))
if (ArraysLoaded==FALSE)
{
Try(tkmessageBox(title="Export HTML Report",message="No arrays have been loaded. Please try New or Open from the File menu.",type="ok",icon="error"))
Try(tkfocus(.limmaGUIglobals$ttMain))
return()
}
if (NumParameterizations>0)
{
Try(parameterizationIndex <- ChooseParameterization())
Try(if (parameterizationIndex==0) return() )
Try(parameterizationTreeIndex <- ParameterizationTreeIndexVec[parameterizationIndex])
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(ComponentsToExport <- GetComponentsToExportInHTMLreport(parameterizationIndex))
}
else
Try(ComponentsToExport <- GetComponentsToExportInHTMLreport())
if (length(ComponentsToExport)==0) return()
Try(fileNameWithPath<- tkgetSaveFile(initialfile=limmaDataSetNameText,filetypes="{{HTML Files} {.html .htm}} {{All files} *}"))
Try(if (nchar(tclvalue(fileNameWithPath))==0)
return())
#Try(path <- tclvalue(tcltk:::tclfile.dir (tclvalue(fileNameWithPath))))
#Try(fileName <- tclvalue(tcltk:::tclfile.tail(tclvalue(fileNameWithPath))))
Try(path <- tclvalue(tclfile.dir (tclvalue(fileNameWithPath))))
Try(fileName <- tclvalue(tclfile.tail(tclvalue(fileNameWithPath))))
###Try(path <- tclvalue(tcl( (tclvalue(fileNameWithPath)),"dir" )))###not working yet - no timee to fix now - do it later
###Try(fileName <- tclvalue(tcl( (tclvalue(fileNameWithPath)),"tail")))###not working yet - no timee to fix now - do it later
Try(len <- nchar(fileName))
if (len<4)
Try(fileName <- paste(fileName,".html",sep=""))
else if ((tolower(substring(fileName,len-4,len))!=".html") &&
(len<5 || (tolower(substring(fileName,len-4,len))!=".html")))
Try(fileName <- paste(fileName,".html",sep=""))
Try(fileNameWithoutExtension <- substring(fileName,1,nchar(fileName)-5))
Try(HTMLfilePath <- paste(path,.Platform$file.sep,fileNameWithoutExtension,"_files",sep=""))
Try(HTMLfileRelativePath <- paste(fileNameWithoutExtension,"_files",sep=""))
Try(dir.create(HTMLfilePath))
Try(fileNameWithPath <- paste(path,"/",fileName,sep=""))
Try(R2HTMLpath <- system.file(package="R2HTML","output"))
Try(cssFileSource <- paste(R2HTMLpath,"/","R2HTML.css",sep=""))
Try(cssFileDestination <- paste(path,"/","R2HTML.css",sep=""))
Try(R2HTMLlogoSource <- paste(R2HTMLpath,"/","R2HTMLlogo.gif",sep=""))
Try(R2HTMLlogoDestination <- paste(path,"/","R2HTMLlogo.gif",sep=""))
Try(file.copy(cssFileSource,cssFileDestination,overwrite=TRUE))
Try(file.copy(R2HTMLlogoSource,R2HTMLlogoDestination,overwrite=TRUE))
Try(HTMLtarget <- HTMLInitFile(path,filename=fileNameWithoutExtension,Title=paste(limmaDataSetNameText,"- Statistical Microarray Analysis using LimmaGUI"), HTMLframe=FALSE,BackGroundColor="#FFFFFF"))
Try(HTML.title(paste(limmaDataSetNameText,"- Statistical Microarray Analysis using LimmaGUI"),HR=1))
Try(ExportTargets <- ComponentsToExport$Targets)
Try(ExportSpotTypes <- ComponentsToExport$SpotTypes)
Try(ExportLayout <- ComponentsToExport$Layout)
Try(ExportBackgroundCorrection <- ComponentsToExport$BackgroundCorrection)
Try(ExportSpotWeighting <- ComponentsToExport$SpotWeighting)
Try(ExportRawMA <- ComponentsToExport$RawMA)
Try(ExportRawPrintTipGroup <- ComponentsToExport$RawPrintTipGroup)
Try(ExportScaleBoxPlot <- ComponentsToExport$ScaleBoxPlot)
Try(ExportSpotTypesInLinearModel <- ComponentsToExport$SpotTypesInLinearModel)
Try(ExportNormalizationInLinearModel <- ComponentsToExport$NormalizationInLinearModel)
Try(ExportDesignMatrix <- ComponentsToExport$DesignMatrix)
Try(ExportDupCor <- ComponentsToExport$DupCor)
Try(ExportTop50Toptables <- ComponentsToExport$Top50Toptables)
Try(ExportCompleteToptables <- ComponentsToExport$CompleteToptables)
Try(ExportAvgMAPlot <- ComponentsToExport$AvgMAPlot)
Try(ExporttStatisticBoxPlots <- ComponentsToExport$tStatisticBoxPlots)
if (ExportRawMA || ExportRawPrintTipGroup || ExportScaleBoxPlot || ExportAvgMAPlot || ExporttStatisticBoxPlots)
{
Try(if (capabilities("png")==FALSE)
Try(tkmessageBox(title="PNG unavailable",message="Your R installation is unable to save PNG images of plots.",icon="warning"))
else
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows" && Sys.info()["sysname"] != "Darwin")
{
Try(pngParams <- GetJpegOrPngX11Params(graphFileType="PNG"))
Try(if (length(pngParams)==0) return())
Try(plotBG <- pngParams$bg)
Try(plotRes <- pngParams$res)
}
else
{
Try(pngParams <- GetJpegOrPngParams(graphFileType="PNG"))
Try(if (length(pngParams)==0) return())
Try(plotWidth <- pngParams$width)
Try(plotHeight <- pngParams$height)
Try(plotPointSize <- pngParams$pointsize)
Try(plotBG <- pngParams$bg)
}))
}
Try(HTML.title("Contents",HR=2))
if (ExportTargets) Try(HTMLli(txt="<a href=\"#Targets\"><b>Targets</b></a>"))
if (ExportSpotTypes) Try(HTMLli(txt="<a href=\"#SpotTypes\"><b>Spot Types</b></a>"))
if (ExportLayout) Try(HTMLli(txt="<a href=\"#Layout\"><b>Layout</b></a>"))
if (ExportBackgroundCorrection) Try(HTMLli(txt="<a href=\"#BackgroundCorrection\"><b>Background Correction</b></a>"))
if (ExportSpotWeighting) Try(HTMLli(txt="<a href=\"#SpotWeighting\"><b>Spot Quality Weighting</b></a>"))
if (ExportRawMA) Try(HTMLli(txt="<a href=\"#RawMA\"><b>Raw M A Plots</b></a>"))
if (ExportRawPrintTipGroup) Try(HTMLli(txt="<a href=\"#RawPrintTipGroup\"><b>Raw Print-Tip Group Loess M A Plots</b></a>"))
if (ExportScaleBoxPlot) Try(HTMLli(txt="<a href=\"#ScaleBoxPlot\"><b>M Box Plot for each Slide</b></a>"))
if (ExportSpotTypesInLinearModel) Try(HTMLli(txt="<a href=\"#SpotTypesInLinearModel\"><b>Spot Types Included In Linear Model</b></a>"))
if (ExportNormalizationInLinearModel) Try(HTMLli(txt="<a href=\"#NormalizationInLinearModel\"><b>Normalization Used In Linear Model</b></a>"))
if (ExportDesignMatrix) Try(HTMLli(txt="<a href=\"#DesignMatrix\"><b>Design Matrix</b></a>"))
if (ExportDupCor) Try(HTMLli(txt="<a href=\"#DupCor\"><b>Duplicate Correlation</b></a>"))
if (ExportTop50Toptables) Try(HTMLli(txt="<a href=\"#Top50Toptables\"><b>Tables of Top 50 Differentially Expressed Genes</b></a>"))
if (ExportCompleteToptables) Try(HTMLli(txt="<a href=\"#CompleteToptables\"><b>Complete Tables of Genes Ranked in order of Evidence for Differential Expression</b></a>"))
if (ExportAvgMAPlot) Try(HTMLli(txt="<a href=\"#AvgMAPlot\"><b>M A Plots (with fitted M values)</b></a>"))
if (ExporttStatisticBoxPlots) Try(HTMLli(txt="<a href=\"#tStatisticBoxPlots\"><b>Box Plots showing the Range of t Statistics for each Spot Type</b></a>"))
Try(tkconfigure(.limmaGUIglobals$ttMain,cursor="watch"))
Try(tkfocus(.limmaGUIglobals$ttMain))
if (ExportTargets)
{
Try(Targets <- get("Targets",envir=limmaGUIenvironment))
Try(ncolTargets <- ncol(Targets))
Try(colnamesTargets <- colnames(Targets))
Try(displayVector <- rep("s",ncolTargets+1))
Try(for (i in (0:ncolTargets))
Try(if (i==0 || colnamesTargets[i]=="SlideNumber")
Try(displayVector[i] <- "d")))
Try(TargetsXtable <- xtable(Targets,display=displayVector))
Try(HTML.title("<a name=\"Targets\">RNA Targets</a>",HR=2))
Try(print(TargetsXtable,type="html",file=fileNameWithPath,append=TRUE))
}
if (ExportSpotTypes)
{
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(SpotTypesXtable <- xtable(SpotTypes))
Try(HTML.title("<a name=\"SpotTypes\">Spot Types</a>",HR=2))
Try(print(SpotTypesXtable,type="html",file=fileNameWithPath,append=TRUE))
}
if (ExportLayout)
{
Try(maLayout <- get("maLayout",envir=limmaGUIenvironment))
Try(layoutDataFrame <- data.frame(NumBlockRows=maLayout$ngrid.r,NumBlockColumns=maLayout$ngrid.c,
NumRowsPerBlock=maLayout$nspot.r,NumColsPerBlock=maLayout$nspot.c))
Try(layoutXtable <- xtable(layoutDataFrame,display=c("d","d","d","d","d")))
Try(HTML.title("<a name=\"Layout\">Layout</a>",HR=2))
Try(print(layoutXtable,type="html",file=fileNameWithPath,append=TRUE))
}
if (ExportBackgroundCorrection)
{
Try(if (!exists("BCMethod",envir=limmaGUIenvironment))
{
Try(BCMethod <- "normexp")
Try(assign("BCMethod",BCMethod,limmaGUIenvironment))
})
Try(BCMethod <- get("BCMethod",envir=limmaGUIenvironment))
Try(HTML.title("<a name=\"BackgroundCorrection\">Background Correction Method</a>",HR=2))
Try(HTMLli(txt=paste("<b>Background Correction Method : </b> ",BCMethod)))
}
if (ExportSpotWeighting)
{
Try(WeightingType <- get("WeightingType",envir=limmaGUIenvironment))
Try(HTML.title("<a name=\"SpotWeighting\">Spot Quality Weighting</a>",HR=2))
Try(HTMLli(txt=paste("<b>Weighting Type : </b> ",WeightingType)))
}
if (ExportRawMA && capabilities("png"))
{
Try(HTML.title("<a name=\"RawMA\">M A Plots Using Raw (Unnormalized) Data</a>",HR=2))
Try(NumSlides <- get("NumSlides",envir=limmaGUIenvironment))
Try(SlideNamesVec <- get("SlideNamesVec",envir=limmaGUIenvironment))
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(MAraw <- get("MAraw",envir=limmaGUIenvironment))
Try(MA.Available <- get("MA.Available",envir=limmaGUIenvironment))
if (MA.Available$Raw)
Try(MAraw <- get("MAraw",envir=limmaGUIenvironment))
else
{
Try (MAraw <- MA.RG(RG))
Try(assign("MAraw",MAraw,limmaGUIenvironment))
Try(MA.Available$Raw <- TRUE)
Try(assign("MA.Available",MA.Available,limmaGUIenvironment))
Try(tkdelete(.limmaGUIglobals$mainTree,"Raw.Status"))
Try(tkinsert(.limmaGUIglobals$mainTree,"end","Raw","Raw.Status" ,text="Available",font=.limmaGUIglobals$limmaGUIfontTree))
}
Try(PointTypes <- SpotTypes$SpotType)
Try(PointColors <- SpotTypes$Color)
Try(numPointTypes <- length(PointTypes))
Try(cex <- rep(0.6,numPointTypes))
Try(for (i in (1:numPointTypes))
if (tolower(PointTypes[i])=="gene"||PointTypes[i]=="cDNA")
{
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows")
cex[i] <- 0.3
else
cex[i] <- 0.1)
})
Try(cex <- cex)
Try(values <- PointTypes)
Try(colVec <- PointColors)
Try(for (slidenum in (1:NumSlides))
{
Try(plotTitle <- paste("M A Scatter Plot for ",SlideNamesVec[slidenum],sep=""))
Try(plotFunction <- function() plotMA(MAraw,pch=16,cex=cex,array=slidenum,
status=SpotTypeStatus,values=values,col=colVec,main=plotTitle,xlab="A",ylab="M"))
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = paste("plotMArawSlide",slidenum,sep=""),
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=plotFunction,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
})
}
if (ExportRawPrintTipGroup && capabilities("png"))
{
Try(HTML.title("<a name=\"RawPrintTipGroup\">Print-Tip Group M A Plots (With Loess Curves) Using Raw (Unnormalized Data)</a>",HR=2))
Try(maLayout <- get("maLayout", envir=limmaGUIenvironment))
Try(NumSlides <- get("NumSlides", envir=limmaGUIenvironment))
Try(SlideNamesVec <- get("SlideNamesVec",envir=limmaGUIenvironment))
Try(MAraw <- get("MAraw", envir=limmaGUIenvironment))
Try(MA.Available <- get("MA.Available",envir=limmaGUIenvironment))
if (MA.Available$Raw)
Try(MAraw <- get("MAraw",envir=limmaGUIenvironment))
else
{
Try (MAraw <- MA.RG(RG))
Try(assign("MAraw",MAraw,limmaGUIenvironment))
Try(MA.Available$Raw <- TRUE)
Try(assign("MA.Available",MA.Available,limmaGUIenvironment))
Try(tkdelete(.limmaGUIglobals$mainTree,"Raw.Status"))
Try(tkinsert(.limmaGUIglobals$mainTree,"end","Raw","Raw.Status" ,text="Available",font=.limmaGUIglobals$limmaGUIfontTree))
}
Try(for (slidenum in (1:NumSlides))
{
Try(plotTitle <- paste("Print-Tip Group Loess M A Plots for ",SlideNamesVec[slidenum],sep=""))
Try(plotFunction <- function() plotPrintTipLoess(MAraw,layout=maLayout,array=slidenum))
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = paste("PrintTipLoessPlotSlide",slidenum,sep=""),
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=plotFunction,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
})
}
if (ExportScaleBoxPlot && capabilities("png"))
{
Try(HTML.title("<a name=\"ScaleBoxPlot\">M Box Plot Showing the Range of M Values for Each Slide</a>",HR=2))
Try(Require("sma"))
Try(RG <- get("RG",envir=limmaGUIenvironment))
Try(maLayout <- get("maLayout", envir=limmaGUIenvironment))
Try(SlideNamesVec <- get("SlideNamesVec",envir=limmaGUIenvironment))
Try(MA.Available <- get("MA.Available",envir=limmaGUIenvironment))
if (MA.Available$WithinArrays)
Try(MA <- get("MAwithinArrays",envir=limmaGUIenvironment))
else
{
Try(if (!exists("WithinArrayNormalizationMethod",envir=limmaGUIenvironment))
{
Try(WithinArrayNormalizationMethod <- "printtiploess")
Try(assign("WithinArrayNormalizationMethod",WithinArrayNormalizationMethod,limmaGUIenvironment))
})
Try(WithinArrayNormalizationMethod <- get("WithinArrayNormalizationMethod",envir=limmaGUIenvironment))
if (WeightingType == "none")
Try (MA <- normalizeWithinArrays(RG,maLayout,method=WithinArrayNormalizationMethod))
else
Try(MA <- normalizeWithinArrays(RG,weights=RG$weights,maLayout,method=WithinArrayNormalizationMethod))
Try(assign("MAwithinArrays",MA,limmaGUIenvironment))
Try(MA.Available$WithinArrays <- TRUE)
Try(assign("MA.Available",MA.Available,limmaGUIenvironment))
Try(tkdelete(.limmaGUIglobals$mainTree,"WithinOnly.Status"))
Try(WithinArrayNormalizationMethod <- get("WithinArrayNormalizationMethod",envir=limmaGUIenvironment))
Try(tkinsert(.limmaGUIglobals$mainTree,"end","WithinOnly","WithinOnly.Status" ,text=paste("Available (using ",WithinArrayNormalizationMethod,")",sep=""),font=.limmaGUIglobals$limmaGUIfontTree))
}
Try(plotTitle <- "M Box Plot for all slides with normalization within arrays only")
Try(plotFunction <- function() boxplot(as.data.frame(MA$M),x.names=SlideNamesVec,xlab="Slide",ylab="M",main=plotTitle))
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = "MScaleBoxPlot",
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=plotFunction,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
}
if (ExportSpotTypesInLinearModel)
{
Try(SpotTypesIncludedNamesVec <- GetSpotTypesIncludedNames(parameterizationTreeIndex))
Try(SpotTypesInLinearModel <- data.frame(SpotTypes=SpotTypesIncludedNamesVec))
Try(colnames(SpotTypesInLinearModel)[1] <- "Spot Types Included In Linear Model")
Try(SpotTypesInLinearModelXtable <- xtable(SpotTypesInLinearModel))
Try(HTML.title("<a name=\"SpotTypesInLinearModel\">Spot Types Included In Linear Model</a>",HR=2))
Try(print(SpotTypesInLinearModelXtable,type="html",file=fileNameWithPath,append=TRUE))
}
if (ExportNormalizationInLinearModel && NumParameterizations > 0)
{
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(WhetherToNormalizeWithinArrays <- (ParameterizationList[[ParameterizationNameNode]])$WhetherToNormalizeWithinArrays)
Try(WhetherToNormalizeBetweenArrays <- (ParameterizationList[[ParameterizationNameNode]])$WhetherToNormalizeBetweenArrays)
Try(HTML.title("<a name=\"NormalizationInLinearModel\">Normalization Used In Linear Model</a>",HR=2))
Try(if (!exists("WithinArrayNormalizationMethod",envir=limmaGUIenvironment))
{
Try(WithinArrayNormalizationMethod <- "printtiploess")
Try(assign("WithinArrayNormalizationMethod",WithinArrayNormalizationMethod,limmaGUIenvironment))
})
Try(WithinArrayNormalizationMethod <- get("WithinArrayNormalizationMethod",envir=limmaGUIenvironment))
Try(if ("WithinArrayNormalizationMethod" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Try(WithinArrayNormalizationMethod <- (ParameterizationList[[ParameterizationNameNode]])$WithinArrayNormalizationMethod)
else
{
Try(ParameterizationList[[ParameterizationNameNode]][[WithinArrayNormalizationMethod]] <- WithinArrayNormalizationMethod)
Try(assign("ParameterizationList",ParameterizationList,limmaGUIenvironment))
})
Try(HTMLli(txt=paste("Within Arrays : ", WhetherToNormalizeWithinArrays," (",WithinArrayNormalizationMethod,")",sep="")))
Try(HTMLli(txt=paste("Between Arrays : ",WhetherToNormalizeBetweenArrays,sep="")))
}
if (ExportDesignMatrix && NumParameterizations > 0)
{
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(designList <- (ParameterizationList[[ParameterizationNameNode]])$designList)
Try(design <- designList$design)
Try(display <- rep("g",ncol(design)))
Try(display <- c("d",display))
Try(designXtable <- xtable(design,display=display))
Try(HTML.title("<a name=\"DesignMatrix\">Design Matrix</a>",HR=2))
Try(print(designXtable,type="html",file=fileNameWithPath,append=TRUE))
}
if (ExportDupCor)
{
Try(ndups <- get("ndups",envir=limmaGUIenvironment))
if (ndups > 1) {
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(dupcor <- (ParameterizationList[[ParameterizationNameNode]])$dupcor)
Try(HTML.title("<a name=\"DupCor\">Duplicate Correlation</a>",HR=2))
Try(HTMLli(txt=paste("Duplicate Correlation :",dupcor$cor)))
}
}
if (ExportTop50Toptables)
{
Try(ndups <- get("ndups",envir=limmaGUIenvironment))
Try(spacing <- GetReducedDuplicateSpacing(parameterizationTreeIndex))
Try(NumParameterizations <- get("NumParameterizations",envir=limmaGUIenvironment))
if (NumParameterizations > 0) {
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(designList <- (ParameterizationList[[ParameterizationNameNode]])$designList)
Try(design <- designList$design)
Try(ParameterizationNamesVec <- get("ParameterizationNamesVec",envir=limmaGUIenvironment))
Try(HTML.title(paste("<a name=\"Top50Toptables\">Top 50 Differentially Expressed Genes for each Parameter in Parameterization ",ParameterizationNamesVec[parameterizationIndex],"</a>",sep=""),HR=2))
Try(NumParameters <- get("NumParameters",envir=limmaGUIenvironment))
Try(if (nrow(design)==0)
{
Try(ParameterNamesVec <- c())
if (NumParameters>0)
for (i in (1:NumParameters))
Try(ParameterNamesVec <- c(ParameterNamesVec,paste("Param",i)))
}
else
Try(ParameterNamesVec <- colnames(design)))
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(Amatrix <- NULL)
Try(fit <- (ParameterizationList[[ParameterizationNameNode]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]])$eb)
Try(if ("Amatrix" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Amatrix <- (ParameterizationList[[ParameterizationNameNode]])$Amatrix)
Try(if (is.null(Amatrix))
{
Try(MA <- get("MA",envir=limmaGUIenvironment))
Try(A <- MA$A)
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(numSpotTypes <- nrow(SpotTypes))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(SpotTypesForLinearModel <- ParameterizationList[[ParameterizationNameNode]]$SpotTypesForLinearModel)
Omit <- ""
count <- 0
Try(for (i in (1:numSpotTypes))
{
if (SpotTypesForLinearModel[i]==TRUE)
next()
count <- count + 1
if (count>1)
Omit <-paste(Omit,"|")
else
Omit <- "("
Try(Omit <- paste(Omit," (SpotTypeStatus==\"",SpotTypes[i,"SpotType"],"\")",sep=""))
})
Try(if (nchar(Omit)>0)
{
Try(Omit <- paste(Omit,")"))
Try(Omit <- eval(parse(text=Omit)))
Try(A <- A[!Omit,])
})
Try(A <- unwrapdups(A,ndups=ndups,spacing=spacing))
}
else
Try(A <- unwrapdups(Amatrix,ndups=ndups,spacing=spacing)))
Try(if ("genelist" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Try(genelist <- (ParameterizationList[[ParameterizationNameNode]])$genelist)
else
Try(genelist <- get("genelist",limmaGUIenvironment)))
for (coef in (1:NumParameters))
{
Try(options(digits=3))
Try(table1 <- toptable(coef=coef,number=50,genelist=genelist,A=A,fit=fit,eb=eb))
Try(toptableDisplay <- rep("s",ncol(table1)+1))
Try(toptableDisplay[1] <- "d")
Try(for (i in (2:(ncol(table1)+1)))
{
Try(if (tolower(colnames(table1)[i-1])=="block") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="column") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="col") toptableDisplay[i] <- "d" )
Try(if (tolower(colnames(table1)[i-1])=="row") toptableDisplay[i] <- "d" )
Try(if (tolower(colnames(table1)[i-1])=="gridrow") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="gridcol") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="gridcolumn") toptableDisplay[i] <- "d")
Try(if (colnames(table1)[i-1]=="M") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="A") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="t") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="P.Value") toptableDisplay[i] <- "e")
Try(if (colnames(table1)[i-1]=="B") toptableDisplay[i] <- "f")
})
# Try(colnames(table1)[ncol(table1)-1] <- sprintf("%-10s",colnames(table1)[ncol(table1)-1]))
Try(toptableXtable <- xtable(table1,display=toptableDisplay))
Try(HTML.title(paste("Top 50 Differentially Expressed Genes for",ParameterNamesVec[coef]),HR=3))
Try(print(toptableXtable,type="html",file=fileNameWithPath,append=TRUE))
}
# Now the Contrasts
Try(NumContrastParameterizations <- ParameterizationList[[ParameterizationNameNode]]$NumContrastParameterizations)
Try(ContrastsParameterizationNamesVec <- c() )
Try(contrastNames <- list())
Try (if (NumContrastParameterizations>0)
Try(for (cp in (1:NumContrastParameterizations))
{
Try(ContrastsParameterizationNamesVec[cp] <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsParameterizationNameText)
Try(contrastsMatrixInList <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsMatrixInList)
Try(contrastsMatrix <- contrastsMatrixInList$contrasts)
Try(contrastNames[[cp]] <- colnames(contrastsMatrix))
Try(fit <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$eb)
for (coef in (1:ncol(contrastsMatrix)))
{
Try(options(digits=3))
Try(table1 <- toptable(coef=coef,number=50,genelist=genelist,A=A,fit=fit,eb=eb))
Try(toptableDisplay <- rep("s",ncol(table1)+1))
Try(toptableDisplay[1] <- "d")
Try(for (i in (2:(ncol(table1)+1)))
{
Try(if (tolower(colnames(table1)[i-1])=="block") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="column") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="col") toptableDisplay[i] <- "d" )
Try(if (tolower(colnames(table1)[i-1])=="row") toptableDisplay[i] <- "d" )
Try(if (tolower(colnames(table1)[i-1])=="gridrow") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="gridcol") toptableDisplay[i] <- "d")
Try(if (tolower(colnames(table1)[i-1])=="gridcolumn") toptableDisplay[i] <- "d")
Try(if (colnames(table1)[i-1]=="M") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="A") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="t") toptableDisplay[i] <- "f")
Try(if (colnames(table1)[i-1]=="P.Value") toptableDisplay[i] <- "e")
Try(if (colnames(table1)[i-1]=="B") toptableDisplay[i] <- "f")
})
# Try(colnames(table1)[ncol(table1)-1] <- sprintf("%-10s",colnames(table1)[ncol(table1)-1]))
Try(toptableXtable <- xtable(table1,display=toptableDisplay))
Try(HTML.title(paste("Top 50 Differentially Expressed Genes for ",colnames(contrastsMatrix)[coef]," [",ContrastsParameterizationNamesVec[cp],"]",sep=""),HR=3))
Try(print(toptableXtable,type="html",file=fileNameWithPath,append=TRUE))
}
}))
}
}
if (ExportCompleteToptables)
{
Try(ndups <- get("ndups",envir=limmaGUIenvironment))
Try(spacing <- GetReducedDuplicateSpacing(parameterizationTreeIndex))
if (NumParameterizations > 0)
{
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(designList <- (ParameterizationList[[ParameterizationNameNode]])$designList)
Try(design <- designList$design)
Try(ParameterizationNamesVec <- get("ParameterizationNamesVec",envir=limmaGUIenvironment))
Try(HTML.title(paste("<a name=\"CompleteToptables\">Complete Tables of Genes Ranked in order of Evidence for Differential Expression for each Parameter in Parameterization ",ParameterizationNamesVec[parameterizationIndex],"</a>",sep=""),HR=2))
Try(NumParameters <- get("NumParameters",envir=limmaGUIenvironment))
Try(if (nrow(design)==0)
{
Try(ParameterNamesVec <- c())
if (NumParameters>0)
for (i in (1:NumParameters))
Try(ParameterNamesVec <- c(ParameterNamesVec,paste("Param",i)))
}
else
Try(ParameterNamesVec <- colnames(design)))
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(Amatrix <- NULL)
Try(if ("Amatrix" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Amatrix <- (ParameterizationList[[ParameterizationNameNode]])$Amatrix)
Try(if (is.null(Amatrix))
{
Try(MA <- get("MA",envir=limmaGUIenvironment))
Try(A <- MA$A)
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(numSpotTypes <- nrow(SpotTypes))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(SpotTypesForLinearModel <- ParameterizationList[[ParameterizationNameNode]]$SpotTypesForLinearModel)
Omit <- ""
count <- 0
Try(for (i in (1:numSpotTypes))
{
if (SpotTypesForLinearModel[i]==TRUE)
next()
count <- count + 1
if (count>1)
Omit <-paste(Omit,"|")
else
Omit <- "("
Try(Omit <- paste(Omit," (SpotTypeStatus==\"",SpotTypes[i,"SpotType"],"\")",sep=""))
})
Try(if (nchar(Omit)>0)
{
Try(Omit <- paste(Omit,")"))
Try(Omit <- eval(parse(text=Omit)))
Try(A <- A[!Omit,])
})
Try(A <- unwrapdups(A,ndups=ndups,spacing=spacing))
}
else
Try(A <- unwrapdups(Amatrix,ndups=ndups,spacing=spacing)))
Try(fit <- (ParameterizationList[[ParameterizationNameNode]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]])$eb)
Try(if ("genelist" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Try(genelist <- (ParameterizationList[[ParameterizationNameNode]])$genelist)
else
Try(genelist <- get("genelist",limmaGUIenvironment)))
for (coef in (1:NumParameters))
{
Try(options(digits=3))
Try(table1 <- toptable(coef=coef,number=nrow(genelist),genelist=genelist,A=A,fit=fit,eb=eb))
# Try(colnames(table1)[ncol(table1)-1] <- sprintf("%-10s",colnames(table1)[ncol(table1)-1]))
Try(ToptableAbsoluteFilename <- paste(HTMLfilePath ,.Platform$file.sep,"CompleteToptable_Param",coef,".xls",sep=""))
Try(ToptableRelativeFilename <- paste(HTMLfileRelativePath ,.Platform$file.sep,"CompleteToptable_Param",coef,".xls",sep=""))
Try(write.table(table1,file=ToptableAbsoluteFilename,quote=FALSE,col.names=NA,sep="\t"))
Try(HTML.title(paste("Complete Table of Genes Ranked in order of Evidence for Differential Expression for ",ParameterNamesVec[coef]),HR=3))
Try(HTMLli(txt=paste("<a href=\"",ToptableRelativeFilename,"\"><b>",paste("CompleteToptable_Param",coef,".xls",sep=""),"</b></a>",sep="")))
}
# Now the Contrasts
Try(NumContrastParameterizations <- ParameterizationList[[ParameterizationNameNode]]$NumContrastParameterizations)
Try(ContrastsParameterizationNamesVec <- c() )
Try(contrastNames <- list())
Try(if (NumContrastParameterizations>0)
Try(for (cp in (1:NumContrastParameterizations))
{
Try(ContrastsParameterizationNamesVec[cp] <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsParameterizationNameText)
Try(contrastsMatrixInList <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsMatrixInList)
Try(contrastsMatrix <- contrastsMatrixInList$contrasts)
Try(contrastNames[[cp]] <- colnames(contrastsMatrix))
Try(fit <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$eb)
for (coef in (1:ncol(contrastsMatrix)))
{
Try(options(digits=3))
Try(table1 <- toptable(coef=coef,number=nrow(genelist),genelist=genelist,A=A,fit=fit,eb=eb))
# Try(colnames(table1)[ncol(table1)-1] <- sprintf("%-10s",colnames(table1)[ncol(table1)-1]))
Try(ToptableAbsoluteFilename <- paste(HTMLfilePath ,.Platform$file.sep,"CompleteToptable_CP_",cp,"Param",coef,".xls",sep=""))
Try(ToptableRelativeFilename <- paste(HTMLfileRelativePath ,.Platform$file.sep,"CompleteToptable_CP_",cp,"Param",coef,".xls",sep=""))
Try(write.table(table1,file=ToptableAbsoluteFilename,quote=FALSE,col.names=NA,sep="\t"))
Try(HTML.title(paste("Complete Table of Genes Ranked in order of Evidence for Differential Expression for ",colnames(contrastsMatrix)[coef]," [",ContrastsParameterizationNamesVec[cp],"]",sep=""),HR=3))
Try(HTMLli(txt=paste("<a href=\"",ToptableRelativeFilename,"\"><b>",paste("CompleteToptable_CP_",cp,"Param",coef,".xls",sep=""),"</b></a>",sep="")))
}
}))
}
}
if (ExportAvgMAPlot && capabilities("png") && NumParameterizations > 0)
{
Try(HTML.title(paste("<a name=\"AvgMAPlot\">M A Plots (with fitted M values) in Parameterization ",ParameterizationNamesVec[parameterizationIndex],"</a>",sep=""),HR=2))
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(designList <- (ParameterizationList[[ParameterizationNameNode]])$designList)
Try(design <- designList$design)
Try(ParameterizationNamesVec <- get("ParameterizationNamesVec",envir=limmaGUIenvironment))
Try(NumParameters <- get("NumParameters",envir=limmaGUIenvironment))
Try(if (nrow(design)==0)
{
Try(ParameterNamesVec <- c())
if (NumParameters>0)
for (i in (1:NumParameters))
Try(ParameterNamesVec <- c(ParameterNamesVec,paste("Param",i)))
}
else
Try(ParameterNamesVec <- colnames(design)))
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(Amatrix <- NULL)
Try(if ("Amatrix" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Amatrix <- (ParameterizationList[[ParameterizationNameNode]])$Amatrix)
Try(if (is.null(Amatrix))
{
Try(MA <- get("MA",envir=limmaGUIenvironment))
Try(A <- MA$A)
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(numSpotTypes <- nrow(SpotTypes))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(SpotTypesForLinearModel <- ParameterizationList[[ParameterizationNameNode]]$SpotTypesForLinearModel)
Omit <- ""
count <- 0
Try(for (i in (1:numSpotTypes))
{
if (SpotTypesForLinearModel[i]==TRUE)
next()
count <- count + 1
if (count>1)
Omit <-paste(Omit,"|")
else
Omit <- "("
Try(Omit <- paste(Omit," (SpotTypeStatus==\"",SpotTypes[i,"SpotType"],"\")",sep=""))
})
Try(if (nchar(Omit)>0)
{
Try(Omit <- paste(Omit,")"))
Try(Omit <- eval(parse(text=Omit)))
Try(A <- A[!Omit,])
})
Try(A <- unwrapdups(A,ndups=ndups,spacing=spacing))
}
else
Try(A <- unwrapdups(Amatrix,ndups=ndups,spacing=spacing)))
Try(meanA <- c())
Try(for (i in (1:nrow(A)))
{
Try(meanA[i] <- mean(A[i,]))
})
Try(A <- meanA)
Try(fit <- (ParameterizationList[[ParameterizationNameNode]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]])$eb)
Try(if ("genelist" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Try(genelist <- (ParameterizationList[[ParameterizationNameNode]])$genelist)
else
Try(genelist <- get("genelist",limmaGUIenvironment)))
for (coef in (1:NumParameters))
{
Try(HTML.title(paste("Average M A Plot showing DE genes for",ParameterNamesVec[coef]),HR=3))
Try(plotTitle <- paste("Average M A Plot showing DE genes for",ParameterNamesVec[coef]))
Try(if (NumParameters>1)
Try(M <- fit$coefficients[,coef])
else
Try(M <- fit$coefficients))
Try(numDEgenesLabeled <- 10)
Try(GeneLabelsMaxLength <- 10)
Try(if (NumParameters>1)
Try(ord <- order(eb$lods[,coef],decreasing=TRUE))
else
Try(ord <- order(eb$lods,decreasing=TRUE)))
Try(topGenes <- ord[1:numDEgenesLabeled])
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows")
Try(cex <- 0.3)
else
Try(cex <- 0.1))
AvgMAPlotFunction <- function()
{
Try(plot(A,M,pch=16,cex=cex,xlab="mean(A)",ylab=paste("M (",ParameterNamesVec[coef],")",sep=""),main=plotTitle))
Try(text(A[topGenes],M[topGenes],labels=substr(genelist[topGenes,"Name"],1,GeneLabelsMaxLength),cex=0.8,col="blue"))
}
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = paste("AvgMAPlot",coef,sep=""),
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=AvgMAPlotFunction,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
}
# Now the Contrasts.
Try(NumContrastParameterizations <- ParameterizationList[[ParameterizationNameNode]]$NumContrastParameterizations)
Try(ContrastsParameterizationNamesVec <- c() )
Try(contrastNames <- list())
Try (if (NumContrastParameterizations>0)
Try(for (cp in (1:NumContrastParameterizations))
{
Try(ContrastsParameterizationNamesVec[cp] <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsParameterizationNameText)
Try(contrastsMatrixInList <- ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]]$contrastsMatrixInList)
Try(contrastsMatrix <- contrastsMatrixInList$contrasts)
Try(contrastNames[[cp]] <- colnames(contrastsMatrix))
Try(fit <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$fit)
Try(eb <- (ParameterizationList[[ParameterizationNameNode]]$Contrasts[[cp]])$eb)
Try(NumContrasts <- ncol(contrastsMatrix))
for (coef in (1:NumContrasts))
{
Try(HTML.title(paste("Average M A Plot showing DE genes for", contrastNames[coef]),HR=3))
Try(plotTitle <- paste("Average M A Plot showing DE genes for",contrastNames[coef]))
Try(if (NumContrasts>1)
Try(M <- fit$coefficients[,coef])
else
Try(M <- fit$coefficients))
Try(numDEgenesLabeled <- 10)
Try(GeneLabelsMaxLength <- 10)
Try(if (NumContrasts>1)
Try(ord <- order(eb$lods[,coef],decreasing=TRUE))
else
Try(ord <- order(eb$lods,decreasing=TRUE)))
Try(topGenes <- ord[1:numDEgenesLabeled])
Try(if (exists("X11", envir=.GlobalEnv) && Sys.info()["sysname"] != "Windows")
Try(cex <- 0.3)
else
Try(cex <- 0.1))
AvgMAPlotFunction2 <- function()
{
Try(plot(A,M,pch=16,cex=cex,xlab="mean(A)",ylab=paste("M (",contrastNames[coef],")",sep=""),main=plotTitle))
Try(text(A[topGenes],M[topGenes],labels=substr(genelist[topGenes,"Name"],1,GeneLabelsMaxLength),cex=0.8,col="blue"))
}
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = paste("AvgMAPlot_CP_",cp,"_",coef,sep=""),
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=AvgMAPlotFunction2,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
}
}))
}
if (ExporttStatisticBoxPlots && capabilities("png") && NumParameterizations > 0)
{
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(designList <- (ParameterizationList[[ParameterizationNameNode]])$designList)
Try(NumParameters <- get("NumParameters",envir=limmaGUIenvironment))
Try(design <- designList$design)
Try(ParameterizationNamesVec <- get("ParameterizationNamesVec",envir=limmaGUIenvironment))
Try(HTML.title(paste("<a name=\"tStatisticBoxPlots\">Box Plots showing the Range of t Statistics for each Spot Type, for each Parameter in Parameterization ",ParameterizationNamesVec[parameterizationIndex],"</a>",sep=""),HR=2))
Try(if (nrow(design)==0)
{
Try(ParameterNamesVec <- c())
if (NumParameters>0)
for (i in (1:NumParameters))
Try(ParameterNamesVec <- c(ParameterNamesVec,paste("Param",i)))
}
else
Try(ParameterNamesVec <- colnames(design)))
Try(ParameterizationList <- get("ParameterizationList",envir=limmaGUIenvironment))
Try(ParameterizationNameNode <- paste("ParameterizationName.",parameterizationTreeIndex,sep=""))
Try(eb <- (ParameterizationList[[ParameterizationNameNode]])$eb)
Try(ndups <- get("ndups",envir=limmaGUIenvironment))
Try(spacing <- get("spacing",envir=limmaGUIenvironment)) # Global version
Try(spacing <- GetReducedDuplicateSpacing(parameterizationTreeIndex))
Try(if ("genelist" %in% attributes(ParameterizationList[[ParameterizationNameNode]])$names)
Try(genelist <- (ParameterizationList[[ParameterizationNameNode]])$genelist)
else
Try(genelist <- get("genelist",limmaGUIenvironment)))
Try(gal <- get("gal",envir=limmaGUIenvironment))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(ebayesStatistic <- "t")
Try(SpotTypesIncludedNamesVec <- GetSpotTypesIncludedNames(parameterizationTreeIndex))
Try(numSpotTypes <- length(SpotTypesIncludedNamesVec))
for (coef in (1:NumParameters))
{
Try(options(digits=3))
Try(HTML.title(paste("Box Plots showing the Range of t Statistics for each Spot Type, for Parameter ",ParameterNamesVec[coef],sep=""),HR=3))
# There is a heading HR=4 in the R2HTML cascaded style sheet.
Try(for (SpotTypeIndex in (1:numSpotTypes))
{
Try(SpotType <- SpotTypesIncludedNamesVec[SpotTypeIndex])
Try(HTML.title(paste("Box Plot(s) showing the Range of t Statistics for each Spot Type, for Parameter ",ParameterNamesVec[coef]," and Spot Type ",SpotType,".",sep=""),HR=3))
Try(gal2 <- cbind(gal,SpotTypeStatus=SpotTypeStatus))
Try(SpotTypes <- get("SpotTypes",envir=limmaGUIenvironment))
Try(numSpotTypes <- nrow(SpotTypes))
Try(SpotTypeStatus <- get("SpotTypeStatus",envir=limmaGUIenvironment))
Try(SpotTypesForLinearModel <- ParameterizationList[[ParameterizationNameNode]]$SpotTypesForLinearModel)
Omit <- ""
count <- 0
Try(for (i in (1:numSpotTypes))
{
if (SpotTypesForLinearModel[i]==TRUE)
next()
count <- count + 1
if (count>1)
Omit <-paste(Omit,"|")
else
Omit <- "("
Try(Omit <- paste(Omit," (SpotTypeStatus==\"",SpotTypes[i,"SpotType"],"\")",sep=""))
})
Try(if (nchar(Omit)>0)
{
Try(Omit <- paste(Omit,")"))
Try(Omit <- eval(parse(text=Omit)))
Try(genelist2 <- uniquegenelist(gal2[!Omit,],ndups=ndups,spacing=spacing))
}
else
Try(genelist2 <- uniquegenelist(gal2,ndups=ndups,spacing=spacing)))
Try(subGeneList <- genelist2[genelist2[,"SpotTypeStatus"]==SpotType,])
Try(t1 <- table((subGeneList[,"Name"])))
Try(SpotSubTypes <- dimnames(t1)[[1]])
Try(numMatches <- length(SpotSubTypes))
if (numMatches==0) next()
Try(if (NumParameters>1)
ebayesStatisticsVector <- eb[[ebayesStatistic]][,coef]
else
ebayesStatisticsVector <- eb[[ebayesStatistic]])
Try(plotTitle <- paste("t Statistic Box Plot(s) for Spot Type",SpotType))
Try(ylabel <- "t Statistic")
Try(if (numMatches<=50)
{
Try(plotCommand <- "boxplot(")
Try(for (i in (1:numMatches))
plotCommand <- paste(plotCommand,"ebayesStatisticsVector[genelist2[,\"Name\"]==\"",SpotSubTypes[i],"\"],",sep=""))
Try(plotCommand <- paste(plotCommand,"names=c(",sep=""))
Try(
if (numMatches>1)
for (i in (1:(numMatches-1)))
plotCommand <- paste(plotCommand,"\"",SpotSubTypes[i],"\"",",",sep=""))
Try(plotCommand <- paste(plotCommand,"\"",SpotSubTypes[numMatches],"\"),",sep=""))
Try(if (numMatches>1)
Try(plotCommand <- paste(plotCommand,"ylab=\"",ylabel,"\",main=plotTitle)",sep=""))
else
Try(plotCommand <- paste(plotCommand,"xlab=\"",SpotType,"\",","ylab=\"",ylabel,"\",main=plotTitle)",sep="")))
}
else
{
Try(plotCommand <- "boxplot(")
Try(plotCommand <- paste(plotCommand,"ebayesStatisticsVector[genelist2[,\"SpotTypeStatus\"]==\"",SpotType,"\"],",sep=""))
Try(plotCommand <- paste(plotCommand,"xlab=\"",SpotType,"\",","names=\"",SpotType,"\",",sep=""))
Try(plotCommand <- paste(plotCommand,"ylab=\"",ylabel,"\",main=plotTitle)",sep=""))
})
# tkmessageBox(message=plotCommand)
plotEbayesBoxPlot <- function()
{
Try(if (numMatches>50)
{
Try(numMatches <- 1)
Try(opar<-par(bg="white",lab=c(numMatches,20,7)))
}
else
Try(opar<-par(bg="white",cex.axis=0.7,las=2,plt=c(0.15,0.9,0.2,0.85),lab=c(numMatches,20,7))))
Try(eval(parse(text=plotCommand)))
Try(tempGraphPar <- par(opar))
Try(grid(NULL,col="navy"))
}
Try(HTMLplotUsingFunction(Caption = plotTitle, File=fileNameWithPath, GraphRelativeDirectory = HTMLfileRelativePath ,
GraphAbsoluteDirectory = HTMLfilePath, GraphFileName = paste("tStatBPsP",coef,"_ST_",SpotTypeIndex,sep=""),
GraphSaveAs = "png", GraphBorder = 1, Align = "left", plotFunction=plotEbayesBoxPlot,
Width=plotWidth,Height=plotHeight,PointSize=plotPointSize,BG=plotBG,res=plotRes))
})
}
}
Try(tkconfigure(.limmaGUIglobals$ttMain,cursor="arrow"))
Try(HTMLhr())
Try(HTMLli(txt="This report was generated by "))
Try(HTMLli(txt=paste("limmaGUI Version",getPackageVersion("limmaGUI"),"(by James Wettenhall), using")))
Try(HTMLli(txt=paste("limma Version",getPackageVersion("limma"),"(by Gordon Smyth),")))
Try(HTMLli(txt=paste("R2HTML Version",getPackageVersion("R2HTML"),"(by Eric Lecoutre) and ")))
Try(HTMLli(txt=paste("xtable Version",getPackageVersion("xtable"),"(by David Dahl)")))
Try(HTMLEndFile())
}
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