pbmcPlate: pbmcPlate Data Set

Description Usage Format Details Source References

Description

One 96-well plate from a BD FACS CAP analysis of Peripheral Blood Mononucleocyte (PMBC) cells.

Usage

1

Format

The format is an object of class flowSet composed of 96 flowFrames. Each flowFrame corresponds to one well from the plate.

Details

BD FACS CAP (http://www.bd.com/technologies/discovery_platform/BD_FACS_CAP.asp) is a platform for screening a large number of antibodies (200+) on human samples. Antibodies are arrayed 3-per well on a 96-well plate, along with the appropriate controls. In this experiment, an early version of FACS CAP was used to screen PBMCs from 2 donors for 189 different human cell surface markers. The complete data set is available from the FICCS site shown below. The pbmcPlate include with plateCore is a lymphocyte enriched subset of one of the replicate plates for donor 1.

Source

Complete dataset available at http://www.ficcs.org/software.html#Data_Files, the Flow Informatics and Computational Cytometry Society website (FICCS)

References

Add reference for plateCore paper (when/if published).


plateCore documentation built on May 6, 2019, 2:41 a.m.