R/helperFunctions.R

In sojourner: Statistical analysis of single molecule trajectories

## helperFunctions
#
################################################################################






##-----------------------------------------------------------------------------
## .timeStamp
## add time stamp and file name as a unique signature of the
## output file @export .timeStamp
.timeStamp=function(filename) {
    
    basename=basename(filename)
    name=unlist(strsplit(basename, split="[.]"))
    fileName=paste(name[1], "-", format(Sys.time(), "%Y%m%d_%H%M%S"), 
        sep="")
    return(fileName)
    
}

##-----------------------------------------------------------------------------
## .valid

# validity check for dt less than track length (-1)
.valid=function(dt, track) {
    
    # get track length of all tracks
    tracklen=dim(track)[1]
    
    if (dt > (tracklen - 1)) {
        stop("\ntrack length:\t", dim(track)[1], "\ndt:\t\t", dt, 
            "\nTime interval (dt) greater than track length-1\n")
    }
}


.valid=function(dt, track) {
    
    # get track length of all tracks
    tracklen=dim(track)[1]
    
    if (dt > (tracklen - 1)) {
        stop("\ntrack length:\t", dim(track)[1], "\ndt:\t\t", dt, 
            "\nTime interval (dt) greater than track length-1\n")
    }
}






##-----------------------------------------------------------------------------
## tracks.msda2sjr

## @export tracks.msda2sjr
tracks.msda2sjr=function(file){

    tracks.file=readMat(file)
    # file.name=basename(file)
    tracks.mat=tracks.file$tracks

    trackl.sjr=lapply(tracks.mat, function(x){
        x=data.frame(x)
        x=x[,-1] # remove time column
        x=x/0.107  # change micrometer to pixel
        colnames(x)=c("x","y")
        x$z=rep(1,times=dim(x)[1])
        return(x)
    })

    trackll.sjr=list(trackl.sjr)
    names(trackll.sjr)=basename(file)
    return(trackll.sjr)

}

# convert msda to sojourner format not used in functions yet

##-----------------------------------------------------------------------------
## same.scale

same.scale=function(mixmdl.lst) {
    
    scale=list()
    length(scale)=length(mixmdl.lst)
    names(scale)=names(mixmdl.lst)
    
    for (i in seq_along(mixmdl.lst)) {
        
        den=density(mixmdl.lst[[i]]$x)
        scale.x=c(min=min(den$x), max=max(den$x))
        scale.y=c(min=min(den$y), max=max(den$y))
        scale[[i]]=data.frame(scale.x, scale.y)
    }
    
    scale.df=do.call(rbind.data.frame, scale)
    scale.same=data.frame(apply(scale.df, 2, function(x) {
        c(min=min(x), max=max(x))
    }))
    
    return(scale.same)
    
}

##-----------------------------------------------------------------------------
## seedIt
## @export seedIt
# seedIt=function(expr,seed){
#     if (is.null(seed)){
#         seed=sample(0:647,1)
#         set.seed(seed)
#     }else{set.seed(seed)}
# 
#     note <- paste("\nRandom number generation seed",seed,"\n")
#     cat(note)
#     structure(expr, seed=seed)
# }
# can't be used according to BiocCheck()
# WARNING: Remove set.seed usage in R code
# https://support.bioconductor.org/p/110439/

##------------------------------------------------------------------------------
## getStartFrame
## getStartFrame returns starting frame of a track/trajectory (using its
## name) at a given index for a track list

# PARAMETERS: track.list,named track list output 
# Note: Last five characters of the original file name without extension (cannot
# contain '.') 
# index, index of the track in the track list (track number)

## @export getStartFrame
getStartFrame=function(track.list, index) {
    return(as.numeric(substr(names(track.list[index]), 
        gregexpr(pattern="\\.", 
        names(track.list[index]))[[1]][1] + 1, gregexpr(pattern="\\.", 
        names(track.list[index]))[[1]][2] - 1)))
}

##-----------------------------------------------------------------------------
## getTrackFileName
## getTrackFileName returns the shortened file name of the track

# PARAMETERS: track.list, named track list output 
# Note: Last five characters of the original file name without extension (cannot
# contain '.')

## @export getTrackFileName
getTrackFileName=function(track.list) {
    return(substr(names(track.list[1]), 1, gregexpr(pattern="\\.", 
        names(track.list[1]))[[1]][1] - 1))
}

##-----------------------------------------------------------------------------
## abTrack
## abTrack returns absolute corrdinates of a track (for plotting)

# PARAMETERS:
# PARAMETERS: track=track input to be transformed into absolute
# coordinates

## @export abTrack
abTrack=function(track) {
    min.x=min(track$x)
    min.y=min(track$y)
    return(data.frame(x=track$x - min.x, y=track$y - min.y))
}
##-----------------------------------------------------------------------------
## removeFrameRecord
## removeFrameRecord remove frame record for backwards compatibility

#PARAMETERS:
# PARAMETERS: track.list=track list with frame record in the fourth
# column

## @export removeFrameRecord
removeFrameRecord=function(track.list) {
    for (i in seq_along(track.list)) {
        track.list[[i]] <- track.list[[i]][-c(4)]
    }
    return(track.list)
}

convert.abtrackll=function(trackll){
    ab.trackll=list()
    for(i in seq_along(trackll)){
        segl=trackll[[i]]
        segl=lapply(trackll[[i]], abTrack)
        ab.trackll[[i]]=segl
    }
    names(ab.trackll)=names(trackll)
    return (ab.trackll)
}

##-----------------------------------------------------------------------------
#TODO: 

# add a one line description to all the helper functions, and maybe output a map
# of function for the helpers as well. it apears all the helpers has been
# exported, so maybe there is no need to sync this function between sojourner
# and sojourner.pro.