W303: Sequence read coverage ratios for wild type S.cerevisiae W303

In Repliscope: Replication Timing Profiling using DNA Copy Number

Sequence read coverage ratios for wild type S.cerevisiae W303

Description

Sequence read coverage ratios for wild type sample (T7107 strain). The cells were stained with DNA dye and sorted based on DNA content into S or G2/M phase fractions. Extracted DNA was sequenced and mapped to sacCer3 genome. Unique reads for replicating (S) and non-replicating (G2/M) samples were calculated in 1 kb genomic bins. The ratio was created by dividing 'score' values from replicating sample by non- replicating sample 'score' values, adjusted by total number of reads.

Usage

data(W303)

Format

data frame with 11350 rows and 7 variables:

chrom

short chromosome name

chromStart

left chromosome coordinate

chromEnd

right chromosome coordinate

name.rep

replicating sample name

name.nonRep

non-replicating sample name

ratio

ratio value in the current bin

ratioFactor

adjustment factor used for the current ratio

Source

S phase sample: SRA; G2 sample: SRA

References

Natsume et al. (2013) Mol Cell 50(5):661-74 (PubMed)

Examples

data(W303)

Repliscope documentation built on Sept. 13, 2022, 9:05 a.m.