Nothing
#' Calculate the melting temperature using the 'Wallace rule'
#'
#' The Wallace rule is often used as rule of thumb for approximate melting temperature calculations for primers with 14 to 20 nt length.
#'
#' @param ntseq Sequence (5' to 3') of one strand of the DNA nucleic acid duplex
#' as string or vector of characters (\strong{Note:} Non-DNA characters are ignored
#' by this method).
#'
#' @param ambiguous Ambiguous bases are taken into account to compute the G and C content when ambiguous is TRUE.
#'
#' @param outlist output a list of Tm and options or only Tm value, default is TRUE.
#'
#' @export
#' @encoding UTF-8
#'
#' @references
#'
#' Thein S L , Lynch J R , Weatherall D J , et al. DIRECT DETECTION OF HAEMOGLOBIN E WITH SYNTHETIC OLIGONUCLEOTIDES[J]. The Lancet, 1986, 327(8472):93.
#'
#' @author
#'
#' Junhui Li
#'
#' @examples
#'
#' ntseq = c('acgtTGCAATGCCGTAWSDBSY') #for wallace rule
#'
#' out <- Tm_Wallace(ntseq,ambiguous = TRUE)
#' out
#' out$Options
#'
#' @export Tm_Wallace
Tm_Wallace <- function (ntseq, ambiguous = FALSE,outlist = TRUE){
mySeq <- check_filter(ntseq, method = "Tm_Wallace")
nSeq <- length(mySeq)
nGC <- nSeq * GC(mySeq, ambiguous = ambiguous,totalnt=TRUE)/100
nAT <- nSeq - nGC
Tm <- 4 * nGC + 2 * nAT
if(outlist==FALSE){
resultList <- as.numeric(Tm)
}else{
resultList <- vector('list',2L)
names(resultList) <- c("Tm","Options")
resultList$Tm <- Tm
resultList$Options <- list("Sequence"=ntseq,"Ambiguous"=ambiguous,"Check filter"=c2s(mySeq),Method="Thein & Wallace 1986")
class(resultList) <- c("TmCalculator","list")
attr(resultList, "nonhidden") <- "Tm"
}
return(resultList)
}
Any scripts or data that you put into this service are public.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.