Nothing
if (interactive()) savehistory();
library("aroma.affymetrix");
log <- Verbose(threshold=-10, timestamp=TRUE);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Settings
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
chipType <- "HuGene-1_0-st-v1";
footer <- list(
createdOn = format(Sys.time(), "%Y%m%d %H:%M:%S", usetz=TRUE),
createdBy = list(
fullname = "Henrik Bengtsson",
email = sprintf("%s@%s", "henrik.bengtsson", "aroma-project.org")
),
srcFiles = list()
);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Get required annotation files
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
cdf <- AffymetrixCdfFile$byChipType(chipType, tags=".*");
print(cdf);
ptb <- AffymetrixProbeTabFile$byChipType(chipType);
print(ptb);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Allocate aroma cell sequence file
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
#acs <- AromaCellSequenceFile$allocateFromCdf(cdf, tags="*,HB20090927");
acs <- AromaCellSequenceFile$allocateFromCdf(cdf, tags="*,HB20120106b");
print(acs);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Import data from the Affymetrix probe-tab file (contains only PMs)
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# There is a tiny fraction of probes with 21 and 23 base pairs;
# 21 23 25
# 1 49 862510
importFrom(acs, ptb, keepSequenceLengths=25, verbose=log);
# Infer MM from PM sequences? Will give an error if no MMs
inferMmFromPm(acs, cdf=cdf, verbose=log); # DOES NOT WORK
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