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# Produces a cghRA.design object from an Agilent TDT file
# Author : Sylvain Mareschal <mareschal@ovsa.fr>
Agilent.design = function(
file,
name = NULL,
organism = as.character(NA),
assembly = as.character(NA),
chromosomes = NULL,
...
) {
# File parsing
dat <- utils::read.table(
file = file,
sep = "\t",
dec = ".",
header = TRUE,
comment.char = "",
quote = NULL,
stringsAsFactors = FALSE
)
# Coordinates
pattern <- "^chr([0-9XxYy]+):([0-9]+)\\-([0-9]+)$"
nameCol <- dat$ID
nameCol[ grepl("^NA\\.[0-9]+$", nameCol) ] <- NA
chrom <- strand <- start <- end <- rep(NA, nrow(dat))
valid <- grepl(dat$ChromosomalLocation, pattern=pattern)
chrom[valid] <- gsub(pattern, "\\1", dat$ChromosomalLocation[valid])
start[valid] <- as.integer(gsub(pattern, "\\2", dat$ChromosomalLocation[valid]))
end[valid] <- as.integer(gsub(pattern, "\\3", dat$ChromosomalLocation[valid]))
strand[valid] <- "+"
# Factorial chrom
if(is.null(chromosomes)) {
chrom <- factor(chrom)
} else {
chrom <- factor(chrom, levels=chromosomes)
}
# Factorial strand
strand <- factor(strand, levels=c("-","+"))
# Default design name
if(is.null(name)) {
name <- sprintf("Agilent %s x %s", max(dat$Row), max(dat$Column))
}
# New object
design <- cghRA.design(
name = dat$ID,
chrom = chrom,
strand = strand,
start = start,
end = end,
id = dat$RefNumber,
control = dat$ControlType,
row = dat$Row,
col = dat$Column,
quality = dat$PerformanceScore,
.name = name,
.organism = organism,
.assembly = assembly
)
return(design)
}
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