Remove debris-contaminated droplets from single-cell based data.
diem requires the following packages to be installed:
diem package also makes use of the ggplot2 and scales packages
Currently, we only support installation of the
diem R package
Check out the vignette for a thorough tutorial on using
Shown below is a quick workflow for reading 10X data, filtering droplets using default parameters, and converting to a seurat object
library(diem) library(Seurat) counts <- read_10x("path/to/10x") sce <- create_SCE(counts) sce <- diem(sce) seur <- convert_to_seurat(sce)
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