nhclust: Cluster a set of neurons

In nat.nblast: NeuroAnatomy Toolbox ('nat') Extension for Assessing Neuron Similarity and Clustering

Cluster a set of neurons

Description

Given an NBLAST all by all score matrix (which may be specified by a package default) and/or a vector of neuron identifiers use hclust to carry out a hierarchical clustering. The default value of the distfun argument will handle square distance matrices and R dist objects.

Usage

nhclust(
  neuron_names,
  method = "ward",
  scoremat = NULL,
  distfun = as.dist,
  ...,
  maxneurons = 4000
)

Arguments

neuron_names	character vector of neuron identifiers.
method	clustering method (default Ward's).
scoremat	score matrix to use (see sub_score_mat for details of default).
distfun	function to convert distance matrix returned by sub_dist_mat into R dist object (default= as.dist).
...	additional parameters passed to hclust.
maxneurons	set this to a sensible value to avoid loading huge (order N^2) distances directly into memory.

Value

An object of class hclust which describes the tree produced by the clustering process.

See Also

hclust, dist

Other scoremats: sub_dist_mat()

Examples

library(nat)
kcscores=nblast_allbyall(kcs20)
hckcs=nhclust(scoremat=kcscores)
# divide hclust object into 3 groups
library(dendroextras)
dkcs=colour_clusters(hckcs, k=3)
# change dendrogram labels to neuron type, extracting this information
# from type column in the metadata data.frame attached to kcs20 neuronlist
labels(dkcs)=with(kcs20[labels(dkcs)], type)
plot(dkcs)
# 3d plot of neurons in those clusters (with matching colours)
open3d()
plot3d(hckcs, k=3, db=kcs20)
# names of neurons in 3 groups
subset(hckcs, k=3)

nat.nblast documentation built on July 9, 2023, 6:12 p.m.