baseline: Align BLI traces with baseline step

In smoke: Small Molecule Octet/BLI Kinetics Experiment

Align BLI traces with baseline step

Description

Align BLI traces with the baseline step right before the association step. The start and end times of baseline are input arguments.

Usage

baseline(obj, tStart, tEnd)

Arguments

obj	obj of "Bli" class
tStart	start time of the baseline
tEnd	end time of the baseline

Details

This step follows doubleBlank step. The output "Bli" object contains the traces aligned with baselines, and the status of "baseline" set to "TRUE". Also the traces number will be checked. If it does not equal the number of ligand concentration, a warning will be raised: "ligand concentrations mis-match trace number".

Value

S4 object of "Bli" class

Author(s)

Qingan Sun, Xiaojun Li, James C Sacchettini

Maintainer: Qingan Sun <[e-mail]>

References

\insertRef

pzasmoke

Examples

bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)

smoke documentation built on May 29, 2024, 8:30 a.m.