smoke-package: Small Molecule Octet/BLI Kinetics Experiment

In smoke: Small Molecule Octet/BLI Kinetics Experiment

Small Molecule Octet/BLI Kinetics Experiment

Description

Bio-Layer Interferometry (BLI) is a technology to determine the binding kinetics between biomolecules. BLI signals are small and noisy when small molecules are investigated as ligands (analytes). We develop this package to process and analyze the BLI data acquired on Octet Red96 from Fortebio more accurately. Sun Q., Li X., et al (2020) <doi:10.1038/s41467-019-14238-3>. In this new version, we organize the BLI experiment data and analysis methods into a S4 class with self-explaining structure.

Details

The DESCRIPTION file: This package was not yet installed at build time.
Index: This package was not yet installed at build time.
'Smoke' is an object-based package to analyze kinetics data from BioLayer Interferometry (BLI). The example dataset was published in Nature Communication 2020, 11: 339.

Author(s)

Qingan Sun, Xiaojun Li, James C Sacchettini

Maintainer: Qingan Sun <[e-mail]>

References

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pzasmoke

Examples

bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)
bli4 <- estimate(bli3)
bli5 <- fitKinetics(bli4)
plotTraces(bli2)
plotKinetics(bli5)
plotResiduals(bli5)

smoke documentation built on May 29, 2024, 8:30 a.m.