estimate: Estimate initial kinetic parameters

In smoke: Small Molecule Octet/BLI Kinetics Experiment

Description

Estimate the initial kinetic parameters, kOn0 and kOff0, and save in the returned "Bli" object. These parameters can serve as the starting value in the next step, fitKinetics, to calculate the kinetics binding model of the small-molecule under investigation.

Usage

estimate(obj)

Arguments

obj	obj of "Bli" class

Value

S4 object of "Bli" class

Note

kOn0 and kOff0 can also be input manually with the setter functions, kOn0<- and kOff0<-.

Author(s)

Qingan Sun, Xiaojun Li, James C Sacchettini

Maintainer: Qingan Sun <[e-mail]>

References

\insertRef

pzasmoke

Examples

load("bli.RData")
bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)
bli4 <- estimate(bli3)

smoke documentation built on Jan. 28, 2020, 5:09 p.m.