mRNAdoubleKDefficiency: mRNA levels for double knock downs

Description Usage Format Source Examples

Description

qPCR measurements for the mRNA level after a double gene knock down (ratio relative to wild type control). The experiments tests the knock down efficiency in the presence of a second gene knock down.

Usage

1

Format

A data frame with 320 observations on the following 5 variables.

template

an ordered factor with levels Fluc < CG10417 < CG13197 < CG9391 < egr < lic < PRL-1 < Rho1 < Tak1

query

a factor with levels CG10417 [query dsRNA] CG13197 [query dsRNA] CG9391 [query dsRNA] egr [query dsRNA] lic [query dsRNA] PRL-1 [query dsRNA] Rho1 [query dsRNA] Tak1 [query dsRNA]

qPCR.target

a factor with levels CG10417 CG13197 CG9391 egr lic PRL-1 Rho1 Tak1

passage

a factor with levels passage 4 passage 42

RNAi

a numeric vector

Source

Horn, Sandmann, Fischer, Huber, Boutros, Mapping of Signalling Networks through Synthetic Genetic Interaction Analysis by RNAi, Nature Methods, 2011, Figure S2.

Examples

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Huber-group-EMBL/RNAinteractMAPK documentation built on Dec. 31, 2020, 1:01 p.m.