Description Usage Arguments Value
The reads_per_index_summary provides a table of the number of mutant and identical reads associated with every index in the data. Identical reads are (obviously) ones with 0 mutations. Sequencer index/reads are when there is 1 mutant out of n reads for an index. RT index/reads are when all reads for an index are mutant.
1 2 3 4 5 6 7 | classify_sequences(
chng,
ident,
reads_per_index_summary,
min_sequencer = 10,
verbose = verbose
)
|
chng |
Set of reads with mutants. |
ident |
Set of reads which are identical. |
reads_per_index_summary |
Table containing how many reads/index are identical/mutant. |
min_sequencer |
Define how stringent one must be to deem a given read/index group as a sequencer-based error. Too small and gets too many false positives, too large and one gets insufficient data to play with. |
verbose |
Print some information while running. |
Groups of reads which are RT, identical, and sequencer; along with summary information.
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