Description Usage Arguments Value Author(s) See Also Examples
read_rna_beatson
reads a file containing promoter annotation data
together with gene expression levels from RNA-Seq experiments using the
fread
function.
1 | read_rna_beatson(file, chr_discarded = NULL, is_GRanges = TRUE)
|
file |
The name of the file to read data values from. |
chr_discarded |
A vector with chromosome names to be discarded. |
is_GRanges |
Logical: if TRUE a GRanges object is returned, otherwise a data.frame object is returned. |
A GRanges
object if is_GRanges
is
TRUE, otherwise a data.table
object.
The GRanges object contains three additional metadata columns:
ensembl_id
: Ensembl IDs of each gene promoter.
gene_name
: Gene name.
gene_fpkm
: Expression level in
FPKM.
These columns can be accessed as follows:
granges_object$ensembl_id
C.A.Kapourani C.A.Kapourani@ed.ac.uk
read_annot_beatson
, read_bs_bismark_cov
1 2 3 | # Get the location of the RNA-Seq file
rna_beatson_file <- system.file("extdata", "rna_beatson.bed", package = "processHTS")
rna_beatson_data <- read_rna_beatson(file=rna_beatson_file, chr_discarded = "chrX", is_GRanges=TRUE)
|
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