NSC_K27M: H3K27me3 ChIP-seq in mouse Neuronal Stem Cell (NSCs)
In benja0x40/Tightrope: ChIP-seq normalization according to background reads density
Description
Usage
Format
Details
References
Description
COUNTS: Precomputed read count matrixes (mm10)
METADATA: Annotation of experimental conditions
SPIKEIN: Number of Drosophila reads (spike-in)
Usage
1
Format
An object of class environment of length 4.
Details
This ChIP-seq dataset was generated by reproducing experiments presented
in our study about the role of the H3.3K27M histone mutation in DIPG
(Mohammad et al., 2017), but adding spike-in with Drosophila chromatin
in the ChIP-seq protocol.
Briefly, H3K27me3 ChIP-seq experiments were performed in mouse NSCs, in
presence or absence of expression of the H3.3K27M histone mutant (K27M)
as well as with or without treatment with EPZ6438 (EPZ), an inhibitor of the
catalytic reaction producing the H3K27me3 histone mark.
References
Mohammad F., Weissmann S., Leblanc B., Pandey D.P. et al.
EZH2 is a potential therapeutic target for H3K27M-mutant pediatric gliomas.
Nature Medicine 2017 http://dx.doi.org/10.1038/nm.4293
benja0x40/Tightrope documentation built on May 24, 2019, 1:35 a.m.
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Description Usage Format Details References
Description
COUNTS: Precomputed read count matrixes (mm10)
METADATA: Annotation of experimental conditions
SPIKEIN: Number of Drosophila reads (spike-in)
Usage
1 |
Format
An object of class environment of length 4.
Details
This ChIP-seq dataset was generated by reproducing experiments presented in our study about the role of the H3.3K27M histone mutation in DIPG (Mohammad et al., 2017), but adding spike-in with Drosophila chromatin in the ChIP-seq protocol. Briefly, H3K27me3 ChIP-seq experiments were performed in mouse NSCs, in presence or absence of expression of the H3.3K27M histone mutant (K27M) as well as with or without treatment with EPZ6438 (EPZ), an inhibitor of the catalytic reaction producing the H3K27me3 histone mark.
References
Mohammad F., Weissmann S., Leblanc B., Pandey D.P. et al. EZH2 is a potential therapeutic target for H3K27M-mutant pediatric gliomas. Nature Medicine 2017 http://dx.doi.org/10.1038/nm.4293
R Package Documentation
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Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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