discover_signatures: Discover mutational signatures

In campbio/musicatk: Mutational Signature Comprehensive Analysis Toolkit

Discover mutational signatures

Description

Mutational signatures and exposures will be discovered using methods such as Latent Dirichlet Allocation (lda) or Non-Negative Matrix Factorization (nmf). These algorithms will deconvolute a matrix of counts for mutation types in each sample to two matrices: 1) a "signature" matrix containing the probability of each mutation type in each sample and 2) an "exposure" matrix containing the estimated counts for each signature in each sample. Before mutational discovery can be performed, variants from samples first need to be stored in a musica object using the create_musica function and mutation count tables need to be created using functions such as build_standard_table.

Usage

discover_signatures(
  musica,
  table_name,
  num_signatures,
  algorithm = "lda",
  seed = 1,
  nstart = 10,
  par_cores = 1
)

Arguments

musica	A musica object.
table_name	Name of the table to use for signature discovery. Needs to be the same name supplied to the table building functions such as build_standard_table.
num_signatures	Number of signatures to discover.
algorithm	Method to use for mutational signature discovery. One of "lda" or "nmf". Default "lda".
seed	Seed to be used for the random number generators in the signature discovery algorithms. Default 1.
nstart	Number of independent random starts used in the mutational signature algorithms. Default 10.
par_cores	Number of parallel cores to use. Only used if method = "nmf". Default 1.

Value

Returns a A musica_result object containing signatures and exposures.

Examples

data(musica)
g <- select_genome("19")
build_standard_table(musica, g, "SBS96", overwrite = TRUE)
discover_signatures(musica = musica, table_name = "SBS96",
num_signatures = 3, algorithm = "lda", seed = 12345, nstart = 1)

campbio/musicatk documentation built on July 14, 2024, 8:28 a.m.