Description Usage Arguments Details Pipeline Parameters References See Also
S4 Class for parameters of a megaptera project pipeline, as
stored in megapteraProj
.
1 |
... |
Arguments in |
The pipeline's verbosity can be fine-tuned with debug.level
:
0 | No progess and diagnostic messages |
1 | Messages on screen |
2 | Messages logged to file |
3 | Messages on screen and logged to file |
4 | Same as 3, in addition current data is saved as .rda object in case of a foreseeable error |
5 | Same as 4, in addition current data is always saved |
data.path
A character string giving the path to the directory there all
data and results will be stored (see megapteraInit
).
gb.seq.download
A character string defining how sequences should be
downloaded from GenBank Nucleotide; can be "eutils"
or "ftp"
.
debug.level
Numeric, a number between 0 and 5, determining the pipeline's verbosity (see Details). (default: 1)
parallel
Logical: if
TRUE
, several steps in the pipeline will be run in parallel,
otherwise all steps are serial.
cpus
Numerical: if
TRUE
, several steps in the pipeline will be run in parallel,
otherwise all steps are serial.
cluster.type
A character
string: if TRUE
, several steps in the pipeline will be run in
parallel, otherwise all steps are serial.
update.seqs
Currently unused.
retmax
Numeric, giving the batch size when downloading sequences from the Entrez History server (default: 500).
max.gi.per.spec
Numeric, giving the maximum number of sequences that will be used per species. Can be used to avoid model organism (e.g., rice, Drosophila, ...) cluttering up the pipeline with thousands of sequences (default: 1000).
max.bp
Numeric, the maximal length of DNA sequences in base pairs to be included in the alignment. The upper limit is determined by the alignment program and the specific alignment and can only be determined by trial-and-error (default: 5000).
reference.max.dist
Currently unused.
min.seqs.reference
Currently unused.
fract.miss
Numeric, ranging between 0 and 1. To avoid long
stretches of only a few sequences at the beginning and the ending of an
alignment block a minimum required number of sequences can be set as a
fraction of the total number of sequences in this alignment block. Has been
superseeded by the gb.*
parameters.
block.max.dist
Numeric, ranging between 0 and 1.
block.max.dist
gives the maximum genetic distance (measured as the
fraction of divergent nucleotide positions) allowed in a sequence alignment
block. The alignment of individual marker is iteratively broken into
smaller blocks until this condition is met with.
min.n.seq
Numeric, the minimum number of sequences required
for an alignment block. Alignment blocks with less than min.n.seq
are dropped from the output.
max.mad
Numeric, giving the
treshold value for the assessment of saturation: alignments with a median
average distance (MAD) of max.mad
or greater will be broken into
blocks. The default value has been estimated with simulation by Smith et
al. (2009).
gb1
Parameters for masking of alignment blocks
with gblocks
.
gb2
Parameters for masking of
alignment blocks with gblocks
.
gb3
Parameters
for masking of alignment blocks with gblocks
.
gb4
Parameters for masking of alignment blocks with
gblocks
.
gb5
Parameters for masking of
alignment blocks with gblocks
.
Smith, S.A., J.M. Beaulieu, and M.J. Donoghue. 2009. Mega-phylogeny approach for comparative biology: an alternative to supertree and supermatrix approaches. BMC Evolutionary Biology 9:37.
megapteraProj
for creating a megaptera project.
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