readDepthBudgetCalculation.restrictedDoublets: Estimate possible read depth depending on the total cost and...

In heiniglab/scPower: Experimental design framework for scRNAseq population studies (eQTL and DE)

Estimate possible read depth depending on the total cost and the other parameters (with a restricted number of cells per lane)

Description

Estimate possible read depth depending on the total cost and the other parameters (with a restricted number of cells per lane)

Usage

readDepthBudgetCalculation.restrictedDoublets(
  nSamples,
  nCells,
  totalCost,
  costKit,
  cellsPerLane,
  costFlowCell,
  readsPerFlowcell,
  reactionsPerKit = 6
)

Arguments

nSamples	Number of samples
nCells	Cells per individual
totalCost	Experimental budget
costKit	Cost for one 10X kit
cellsPerLane	Number of cells sequenced per lane
costFlowCell	Cost of one flow cells for sequencing
readsPerFlowcell	Number reads that can be sequenced with one flow cell
reactionsPerKit	Reactions (=lanes) per kit, defines the total number of tested individuals per kit

Value

Read depth that can be measured with this budget and other parameters

heiniglab/scPower documentation built on Jan. 9, 2025, 12:13 p.m.